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JoVE Journal
Bioengineering

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FIBS-enabled Noninvasive Metabolic Profiling
 

FIBS-enabled Noninvasive Metabolic Profiling

Article DOI: 10.3791/51200
February 3rd, 2014

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Summary February 3rd, 2014

A description of how to calibrate Förster Resonance Energy Transfer integrated biological sensors (FIBS) for in situ metabolic profiling is presented. The FIBS can be used to measure intracellular levels of metabolites noninvasively aiding in the development of metabolic models and high throughput screening of bioprocess conditions.

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