rapid-analysis-and-exploration-of-fluorescence-microscopy-images

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Despite rapid advances in high-throughput microscopy, quantitative image-based assays still pose significant challenges. While a variety of specialized ...

generation-human-motor-units-with-functional-neuromuscular-junctions

Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices

Neuromuscular junctions (NMJs) are specialized synapses between the axon of the lower motor neuron and the muscle facilitating the engagement of muscle ...

UT Southwestern Medical Center

KU Leuven - University of Leuven

University Hospitals Leuven

Cell polarity is involved in many biological functions such as development, wound healing and immune responses. In human neutrophils, polarization is characterized by the translocation of distinct sets of signaling molecules to opposite ends of the cell and the rapid rearrangement of cytoskeleton to initiate migration. While many image-based studies have described cellular morphology and the intensity level of polarity signaling molecules, systematic characterization of the spatial distribution of polarity signaling molecules has been lacking. Here we designed a collection of analytical features to quantify spatial phenotypes of polarity molecules. We compared our features to commonly used polarity readouts and found that they captured additional aspects of the polarization dynamics that were not contained in the existing features. Our work provides a starting point to identify informative features for the study of neutrophil polarization.

ON IDENTIFYING INFORMATION FROM IMAGE-BASED SPATIAL POLARITY PHENOTYPES IN NEUTROPHILS.

SimuCell&#xFF1A; &#x4E00;&#x4E2A;&#x7075;&#x6D3B;&#x7684;&#x6846;&#x67B6;&#x7528;&#x6765;&#x521B;&#x5EFA;&#x5408;&#x6210;&#x663E;&#x5FAE;&#x955C;&#x56FE;&#x50CF;&#x3002;

SimuCell: a flexible framework for creating synthetic microscopy images.

SimuCell : un cadre souple pour cr&eacute;er des images de microscopie synth&eacute;tique.

SimuCell: einen flexiblen Rahmen f&uuml;r die Erstellung von synthetischen Mikroskopie-Bildern.

SimuCell: un quadro flessibile per la creazione di immagini di microscopia sintetico.

SimuCell: &#x5408;&#x6210;&#x9855;&#x5FAE;&#x93E1;&#x753B;&#x50CF;&#x3092;&#x4F5C;&#x6210;&#x3059;&#x308B;&#x67D4;&#x8EDF;&#x306A;&#x30D5;&#x30EC;&#x30FC;&#x30E0;&#x30EF;&#x30FC;&#x30AF;&#x3002;

SimuCell: &#xAC00;&#xC0C1; &#xD604;&#xBBF8;&#xACBD; &#xC774;&#xBBF8;&#xC9C0;&#xB97C; &#xB9CC;&#xB4E4;&#xAE30; &#xC704;&#xD55C; &#xC720;&#xC5F0;&#xD55C; &#xD504;&#xB808;&#xC784; &#xC6CC;&#xD06C;.

SimuCell: un marco flexible para la creaci&oacute;n de im&aacute;genes sint&eacute;ticas de la microscopia.

PhenoRipper&#xFF1A; &#x8F6F;&#x4EF6;&#x8FDB;&#x884C;&#x5FEB;&#x901F;&#x5206;&#x6790;&#x7684;&#x663E;&#x5FAE;&#x955C;&#x56FE;&#x50CF;&#x3002;

PhenoRipper: software for rapidly profiling microscopy images.

PhenoRipper : logiciel de profilage rapidement des images de microscopie.

PhenoRipper: Software f&uuml;r schnelle Profilerstellung Mikroskopie-Bildern.

PhenoRipper: software per la profilatura rapidamente immagini di microscopia.

PhenoRipper: &#x6025;&#x901F;&#x306B;&#x9855;&#x5FAE;&#x93E1;&#x753B;&#x50CF;&#x3092;&#x30D7;&#x30ED;&#x30D5;&#x30A1;&#x30A4;&#x30EA;&#x30F3;&#x30B0;&#x7528;&#x30BD;&#x30D5;&#x30C8;&#x30A6;&#x30A7;&#x30A2;&#x3002;

&#xD604;&#xBBF8;&#xACBD; &#xC774;&#xBBF8;&#xC9C0;&#xB97C; &#xBE60;&#xB974;&#xAC8C; &#xD504;&#xB85C; &#xD30C;&#xC77C;&#xB9C1; PhenoRipper: &#xC18C;&#xD504;&#xD2B8;&#xC6E8;&#xC5B4;&#xC785;&#xB2C8;&#xB2E4;.

PhenoRipper: software para perfilar r&aacute;pidamente im&aacute;genes de microscop&iacute;a.

ArticleTotal : 2	Year
Publication title Cited by 3	2014
Publication title Cited by 12	2021

Article	Year
ON IDENTIFYING INFORMATION FROM IMAGE-BASED SPATIAL POLARITY PHENOTYPES IN NEUTROPHILS. Proceedings / IEEE International Symposium on Biomedical Imaging: from nano to macro. IEEE International Symposium on Biomedical Imaging\| PubMed ID: 20725643	2010
SimuCell: a flexible framework for creating synthetic microscopy images. Nature methods\| PubMed ID: 22743763	2012
PhenoRipper: software for rapidly profiling microscopy images. Nature methods\| PubMed ID: 22743764	2012

Benjamin Pavie

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