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Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
This content is Free Access.
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute,KU Leuven – University of Leuven, 2VIB Bio Imaging Core,KU Leuven – University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology,KU Leuven – University of Leuven, 4Department of Neurology,University Hospitals Leuven
Chapters
- 00:05Introduction
- 00:54Pre-Assembled Microfluidic Device Preparation
- 02:39Silicone Microfluidic Device Preparation
- 05:18Neural Progenitor Cell Plating in Microfluidic Devices
- 07:15Human Primary Mesoangioblast (MAB) Plating in Microfluidic Devices
- 08:32Results: Generation of NMJs in Microfluidic Devices
- 10:10Conclusion
We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.
