harmonic-nanoparticles-for-regenerative-research

Harmonic Nanoparticles for Regenerative Research

In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation ...

University of Geneva

Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multiphoton microscopy. We present for the first time a complete survey of the properties of five nanomaterials (KNbO(3), LiNbO(3), BaTiO(3), KTP, and ZnO), describing their preparation and stabilization and providing quantitative estimations of their nonlinear optical response. In the light of their prospective use as biological and clinical markers, we assess their biocompatibility on human healthy and cancerous cell lines. Finally, we demonstrate the great potential for cell imaging of these inherently nonlinear probes in terms of optical contrast, wavelength flexibility, and signal photostability.

Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility.

Potassium niobate nonlinear nanoparticles are used for the first time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength flexibility. The potential of this approach is made evident for tissue-regeneration studies and applications, by capturing a high-speed movie of ESC-derived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.

High-speed tracking of murine cardiac stem cells by harmonic nanodoublers.

A biophotonics approach based on the nonlinear optical process of second harmonic generation is presented and demonstrated on malignant human cell lines labelled by harmonic nanoparticles. The method enables independent imaging and therapeutic action, selecting each modality by simply tuning the excitation laser wavelength from infrared to visible. In particular, the generation of deep ultraviolet radiation at 270 nm allows direct interaction with nuclear DNA in the absence of photosensitizing molecules.

Article	Year
Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility. ACS nano\| PubMed ID: 22324660	2012
High-speed tracking of murine cardiac stem cells by harmonic nanodoublers. Small (Weinheim an der Bergstrasse, Germany)\| PubMed ID: 22859385	2012
Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples. Nanoscale\| PubMed ID: 24477750	2014

Thibaud Magouroux

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