Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
Ronzoni, F., Magouroux, T., Vernet, R., Extermann, J., Crotty, D., Prina-Mello, A., Ciepielewski, D., Volkov, Y., Bonacina, L., Wolf, J., Jaconi, M. Harmonic Nanoparticles for Regenerative Research. J. Vis. Exp. (87), e51333, doi:10.3791/51333 (2014).