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Harmonic Nanoparticles for Regenerative Research
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JoVE Journal Bioengineering
Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

DOI:
10.3791/51333-v

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09:23 min

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May 01, 2014

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, , , , , , , , , ,
1Department of Pathology and Immunology, Faculty of Medicine,University of Geneva, 2Physics Department, GAP-Biophotonics,University of Geneva, 3Laboratoire d’Optique Biomédicale (LOB), Faculté des Sciences et Techniques de l’Ingénieur,École Polytechnique Fédérale de Lausanne, 4Department of Clinical Medicine, School of Medicine,Trinity College Dublin, 5School of Medicine and CRANN,Trinity College Dublin, 6Nikon AG Instruments

Chapters

  • 00:05Title
  • 02:26Human ESC Differentiation Protocol
  • 03:50Air-liquid 3D Cultures of Beating Clusters, Labeling with SHG-NPs, and Non-linear Optical Imaging
  • 05:52Results: Assessing Cardiac Activity of SHG-NP-labeled Cardiac Clusters
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.

Tags

Keywords: Harmonic NanoparticlesSecond Harmonic GenerationHuman Embryonic Stem CellsMulti-photon ImagingCardiac Differentiation3D MonitoringSecond Harmonic Emission3D Displacement Patterns

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