high-yield-purification-plasmodium-falciparum-merozoites-for-use

High Yield Purification of <em>Plasmodium falciparum</em> Merozoites For Use in Opsonizing Antibody Assays

Plasmodium falciparum merozoite antigens are under development as potential malaria vaccines. One aspect of immunity against malaria is the removal of ...

Walter and Eliza Hall Institute of Medical Research

University of Melbourne

Targeting merozoiti gli anticorpi sono importanti nella protezione dalla malaria. Pertanto, le proteine di superficie merozoite sono candidati vaccinali attraente. C&#39;&egrave; bisogno di analisi funzionale robuste indagare i meccanismi dell&#39;immunit&agrave; acquisita e l&#39;efficacia del vaccino. Ad oggi, lo studio della fagocitosi merozoite ha stato confuso dalla complessit&agrave; e variabilit&agrave; dei saggi in vitro.

Efficace misura di Opsonising anticorpi a Plasmodium falciparum merozoiti.

Anticorps ciblant les m&eacute;rozo&iuml;tes sont importantes dans la protection contre le paludisme. Prot&eacute;ines de surface m&eacute;rozo&iuml;te sont donc attractifs vaccins candidats. Il y a un besoin pour des tests fonctionnels robustes &eacute;tudier les m&eacute;canismes de l&#39;immunit&eacute; acquise et de l&#39;efficacit&eacute; du vaccin. A ce jour, l&#39;&eacute;tude de la phagocytose m&eacute;rozo&iuml;te a &eacute;t&eacute; confondu par la complexit&eacute; et la variabilit&eacute; des essais in vitro.

Efficace mesure de Opsonising anticorps &agrave; Plasmodium falciparum m&eacute;rozo&iuml;tes.

Antik&ouml;rper, die gezielt Merozoiten sind wichtige Schutz vor Malaria. Merozoite Oberfl&auml;che Proteine sind daher attraktive Impfstoff Kandidaten. Besteht ein Bedarf f&uuml;r robuste funktionelle Assays, Mechanismen der erworbenen Immunit&auml;t und Impfstoff Wirksamkeit zu untersuchen. Bis heute hat das Studium der Merozoite Phagozytose durch die Komplexit&auml;t und Variabilit&auml;t von in-vitro Assays vermischt wurden.

Effiziente Messung der Opsonising Antik&ouml;rper gegen Plasmodium Falciparum Merozoiten.

&#x9488;&#x5BF9; merozoites &#x7684;&#x6297;&#x4F53;&#x4E5F;&#x4ECE;&#x759F;&#x75BE;&#x4FDD;&#x62A4;&#x5F88;&#x91CD;&#x8981;&#x3002;&#x56E0;&#x6B64;&#xFF0C;&#x88C2;&#x6B96;&#x5B50;&#x8868;&#x9762;&#x86CB;&#x767D;&#x662F;&#x6709;&#x5438;&#x5F15;&#x529B;&#x7684;&#x75AB;&#x82D7;&#x5019;&#x9009;&#x4EBA;&#x3002;&#x6709;&#x9700;&#x8981;&#x5F3A;&#x5927;&#x7684;&#x529F;&#x80FD;&#x6027;&#x5206;&#x6790;&#x8C03;&#x67E5;&#x83B7;&#x5F97;&#x6027;&#x514D;&#x75AB;&#x548C;&#x75AB;&#x82D7;&#x6548;&#x529B;&#x7684;&#x673A;&#x5236;&#x3002;&#x5230;&#x76EE;&#x524D;&#x4E3A;&#x6B62;&#xFF0C;&#x88C2;&#x6B96;&#x5B50;&#x541E;&#x566C;&#x529F;&#x80FD;&#x7684;&#x7814;&#x7A76;&#x5DF2;&#x7ECF;&#x6253;&#x4E71;&#x4E86;&#x7684;&#x590D;&#x6742;&#x6027;&#x548C;&#x53EF;&#x53D8;&#x6027;&#x7684;&#x4F53;&#x5916;&#x68C0;&#x6D4B;&#x65B9;&#x6CD5;&#x3002;

&#x6709;&#x6548;&#x6D4B;&#x91CF;&#x7684; Opsonising &#x6297;&#x4F53;&#x7684;&#x6076;&#x6027;&#x759F;&#x539F;&#x866B; Merozoites&#x3002;

Antibodies targeting merozoites are important in protection from malaria. Therefore, merozoite surface proteins are attractive vaccine candidates. There is a need for robust functional assays to investigate mechanisms of acquired immunity and vaccine efficacy. To date, the study of merozoite phagocytosis has been confounded by the complexity and variability of in vitro assays.

Efficient Measurement of Opsonising Antibodies to Plasmodium falciparum Merozoites.

&#x30E1;&#x30ED;&#x30BE;&#x30A4;&#x30C8; &#x30BF;&#x30FC;&#x30B2;&#x30C3;&#x30C8;&#x6297;&#x4F53;&#x306F;&#x30DE;&#x30E9;&#x30EA;&#x30A2;&#x304B;&#x3089;&#x306E;&#x4FDD;&#x8B77;&#x306B;&#x91CD;&#x8981;&#x3067;&#x3059;&#x3002;&#x3057;&#x305F;&#x304C;&#x3063;&#x3066;&#x3001;, &#x30E1;&#x30ED;&#x30BE;&#x30A4;&#x30C8;&#x8868;&#x9762;&#x86CB;&#x767D;&#x8CEA;&#x9B45;&#x529B;&#x7684;&#x306A;&#x30EF;&#x30AF;&#x30C1;&#x30F3;&#x5019;&#x88DC;&#x3067;&#x3059;&#x3002;&#x7372;&#x5F97;&#x514D;&#x75AB;&#x3068;&#x30EF;&#x30AF;&#x30C1;&#x30F3;&#x306E;&#x6709;&#x52B9;&#x6027;&#x306E;&#x30E1;&#x30AB;&#x30CB;&#x30BA;&#x30E0;&#x3092;&#x8ABF;&#x67FB;&#x3059;&#x308B;&#x305F;&#x3081;&#x306B;&#x5805;&#x7262;&#x306A;&#x6A5F;&#x80FD;&#x30A2;&#x30C3;&#x30BB;&#x30A4;&#x304C;&#x5FC5;&#x8981;&#x3067;&#x3059;&#x3002;&#x65E5;&#x4ED8;&#x3059;&#x308B;&#x306B;&#x306F;&#x3001;&#x8907;&#x96D1;&#x3055;&#x3068; in vitro &#x30A2;&#x30C3;&#x30BB;&#x30A4;&#x306E;&#x5909;&#x52D5;&#x306B;&#x3088;&#x3063;&#x3066;&#x30E1;&#x30ED;&#x30BE;&#x30A4;&#x30C8;&#x8CAA;&#x98DF;&#x80FD;&#x306E;&#x691C;&#x8A0E;&#x3092;&#x56F0;&#x60D1;&#x3057;&#x3066;&#x3044;&#x307E;&#x3059;&#x3002;

&#x52B9;&#x7387;&#x7684;&#x306A;&#x6E2C;&#x5B9A;&#x306E; Opsonising &#x3078;&#x306E;&#x6297;&#x4F53;&#x71B1;&#x5E2F;&#x71B1;&#x30DE;&#x30E9;&#x30EA;&#x30A2;&#x539F;&#x866B;&#x30E1;&#x30ED;&#x30BE;&#x30A4;&#x30C8;&#x3002;

Merozoites&#xC744; &#xB300;&#xC0C1;&#xC73C;&#xB85C; &#xD558;&#xB294; &#xD56D; &#xCCB4;&#xB294; &#xB9D0;&#xB77C;&#xB9AC;&#xC544; &#xB85C;&#xBD80;&#xD130; &#xBCF4;&#xD638;&#xC5D0; &#xC911;&#xC694; &#xD558;&#xB2E4;. &#xB530;&#xB77C;&#xC11C; merozoite &#xD45C;&#xBA74; &#xB2E8;&#xBC31;&#xC9C8;&#xC740; &#xB9E4;&#xB825;&#xC801;&#xC778; &#xBC31;&#xC2E0; &#xD6C4;&#xBCF4;. &#xD6C4;&#xCC9C;&#xC131; &#xBA74;&#xC5ED;&#xACFC; &#xBC31;&#xC2E0; &#xD6A8;&#xB2A5;&#xC758; &#xBA54;&#xCEE4;&#xB2C8;&#xC998;&#xC744; &#xC870;&#xC0AC; &#xD558;&#xAE30; &#xC704;&#xD574; &#xAC15;&#xB825;&#xD55C; &#xAE30;&#xB2A5; &#xBD84;&#xC11D; &#xC2E4;&#xD5D8;&#xC5D0; &#xB300; &#xD55C; &#xD544;&#xC694;&#xAC00; &#xC788;&#xB2E4;. &#xC9C0;&#xAE08;&#xAE4C;&#xC9C0; merozoite &#xC2DD; &#xADE0; &#xC791;&#xC6A9;&#xC758; &#xC5F0;&#xAD6C; &#xBCF5;&#xC7A1;&#xC131;&#xACFC; &#xC0DD;&#xCCB4; &#xC678; &#xBD84;&#xC11D; &#xC2E4;&#xD5D8;&#xC758; &#xBCC0;&#xD654;&#xC5D0; &#xC758;&#xD574; &#xD63C;&#xB3D9; &#xD588;&#xB2E4; &#xB418;&#xC5C8;&#xC2B5;&#xB2C8;&#xB2E4;.

&#xD6A8;&#xC728;&#xC801;&#xC778; &#xCE21;&#xC815;&#xC758; Opsonising&#xC5D0;&#xB294; &#xD56D; &#xCCB4; Plasmodium falciparum Merozoites.

Anticuerpos dirigidos a merozoitos son importantes en la protecci&oacute;n contra la malaria. Por lo tanto, prote&iacute;nas de la superficie merozoito son candidatos vacunales atractivo. Hay una necesidad de an&aacute;lisis funcionales robustas investigar los mecanismos de la inmunidad adquirida y la eficacia de la vacuna. Hasta la fecha, el estudio de la fagocitosis merozoito ha sido confundido por la complejidad y variabilidad de ensayos in vitro.

Eficiente medici&oacute;n de Opsonising anticuerpos al Plasmodium falciparum merozoitos.

Naturally acquired humoral immunity to the malarial parasite Plasmodium falciparum can protect against disease, although the precise mechanisms remain unclear. Although antibody levels can be measured by ELISA, few studies have investigated functional antibody assays in relation to clinical outcomes. In this study we applied a recently developed functional assay of antibody-mediated opsonisation of merozoites, to plasma samples from a longitudinal cohort study conducted in a malaria endemic region of Papua New Guinea (PNG). Phagocytic activity was quantified by flow cytometry using a standardized and high-throughput protocol, and was subsequently evaluated for association with protection from clinical malaria and high-density parasitemia. Opsonising antibody responses were found to: i) increase with age, ii) be enhanced by concurrent infection, and iii) correlate with protection from clinical episodes and high-density parasitemia. Stronger protective associations were observed in individuals with no detectable parasitemia at baseline. This study presents the first evidence for merozoite phagocytosis as a correlate of acquired immunity and clinical protection against P. falciparum malaria.

Article	Year
Efficient Measurement of Opsonising Antibodies to Plasmodium falciparum Merozoites. PloS one\| PubMed ID: 23300556	2012
Opsonising antibodies to P. falciparum merozoites associated with immunity to clinical malaria. PloS one\| PubMed ID: 24040299	2013

Danika L. Hill

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