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Immunology and Infection

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JoVE Journal Immunology and Infection
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
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High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

Article DOI: 10.3791/51590-v • 10:38 min • July 17th, 2014
July 17th, 2014 •
1,2, 1,2, 1,2
1Division of Infection and Immunity, Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology, University of Melbourne

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Summary
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Measuring antibody function is key to understanding immunity to Plasmodium falciparum malaria. This method describes the purification of viable merozoites, and measurement of opsonization-dependent phagocytosis by flow cytometry.

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Plasmodium Falciparum Merozoites Opsonizing Antibody Assays Purification High Yield Malaria Vaccines Phagocytic Cells E64 Protease Inhibitor Schizont Rupture Pro-monocytic Cell Line THP-1 Ethidium Bromide Human Plasma Samples High Throughput Protocol Immune Function Clinical Immunity Vaccine-induced Antibodies
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