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High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

DOI:
10.3791/51590-v

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10:38 min

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July 17, 2014

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, ,
1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

Chapters

  • 00:05Title
  • 01:34Isolation of P. falciparum Merozoites
  • 04:42Quantifying Merozoite Concentration by Flow Cytometry
  • 06:03Phagocytosis Assay
  • 07:48Results: The Merzoite:THP-1 Ratio Influences the Degree of Phagocytosis Observed
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

Measuring antibody function is key to understanding immunity to Plasmodium falciparum malaria. This method describes the purification of viable merozoites, and measurement of opsonization-dependent phagocytosis by flow cytometry.

Tags

Keywords: Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

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