transfecting-raw2647-cells-with-a-luciferase-reporter-gene

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Transfection of desired genetic materials into cells is an inevitable procedure in biomedical research studies. While numerous methods have been ...

Vancouver Costal Health Research Institute

University of British Columbia

A new paradigm in phosphoinositide signaling? (Comment on DOI 10.1002/bies.201100195).

The anti-inflammatory cytokine interleukin-10 (IL-10) is essential for attenuating the inflammatory response, which includes reducing the expression of pro-inflammatory microRNA-155 (miR-155) in lipopolysaccharide (LPS) activated macrophages. miR-155 enhances the expression of pro-inflammatory cytokines such as TNFα and suppresses expression of anti-inflammatory molecules such as SOCS1. Therefore, we examined the mechanism by which IL-10 inhibits miR-155. We found that IL-10 treatment did not affect the transcription of the miR-155 host gene nor the nuclear export of pre-miR-155, but rather destabilized both pri-miR-155 and pre-miR-155 transcripts, as well as interfered with the final maturation of miR-155. This inhibitory effect of IL-10 on miR-155 expression involved the contribution of both the STAT3 transcription factor and the phosphoinositol phosphatase SHIP1. This is the first report showing evidence that IL-10 regulates miRNA expression post-transcriptionally.

Article	Year
A new paradigm in phosphoinositide signaling? (Comment on DOI 10.1002/bies.201100195). BioEssays : news and reviews in molecular, cellular and developmental biology\| PubMed ID: 22791387	2012
Interleukin-10 inhibits lipopolysaccharide induced miR-155 precursor stability and maturation. PloS one\| PubMed ID: 23951138	2013

Eva Y. So

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