polymeric-microneedle-array-fabrication-by-photolithography

Polymeric Microneedle Array Fabrication by Photolithography

This manuscript describes the fabrication of polymeric microneedle (MN) arrays by photolithography. It involves a simple mold-free process by using a ...

National University of Singapore

Raloxifene HCl (RLX) shows low oral bioavailability (<2%) in humans due to poor aqueous solubility and extensive (>90%) metabolism in gut. Lipid nanoparticles (SLN) with glyceryl tribehenate were designed to enhance drug's oral bioavailability. Box-Bhenken design was used to optimize manufacturing conditions. Optimized SLN had particle size of 167±3nm and high encapsulation efficiency (>92%). Oral bioavailability of RLX from SLN was improved by 3.24 folds compared to free RLX in female Wistar rats. Both clathrin and caveolae mediated endocytosis pathways were involved in the uptake of SLN. Lymphatic transport inhibitor, cycloheximide significantly reduced oral bioavailability of SLN.

Lipid nanoparticles for oral delivery of raloxifene: optimization, stability, in vivo evaluation and uptake mechanism.

A photomask consisting plano-convex microlenses for the production of polymeric microneedles was fabricated from a microinjection array. The microinjection array was first fabricated using photolithographical approach and subsequently assembled onto a polydimethylsiloxane (PDMS) stamp. Poly (ethylene glycol) diacrylate (PEGDA) solution was loaded into the microinjection stamp. The microinjection stamp was then applied onto a coverslip to dispense the polymer solution, producing liquid microdroplets. They were then irradiated to form plano-convex microlenses. These microlenses were evaluated for their geometric properties and were fabricated into photomasks. The photomask consisting microlenses was used to fabricate polymeric microneedles that were evaluated and tested for skin penetration efficiency.

Article	Year
Lipid nanoparticles for oral delivery of raloxifene: optimization, stability, in vivo evaluation and uptake mechanism. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V\| PubMed ID: 24378615	2014
Fabrication of photomasks consisting microlenses for the production of polymeric microneedle array. Drug delivery and translational research\| PubMed ID: 26208649	2015

Himanshu Kathuria

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