easy-and-accurate-mechano-profiling-on-micropost-arrays

Easy and Accurate Mechano-profiling on Micropost Arrays

Cell culture substrates with integrated flexible microposts enable a user to study the mechanical interactions between cells and their immediate ...

Balgrist University Hospital

A continuous transport process for liquids in micro-channels is reported. Flow was generated by evaporation at the channel end plus capillary forces. The micro-channels integrated into a two-glass-layer device were 110 microm wide, 28 microm deep and 4 or 10 cm long. A continuous liquid transport velocity of up to 2.25 mm s(-1) was observed for aqueous solutions. The flow velocity is shown to increase when an air stream is guided over the evaporation zone.

Evaporation driven pumping for chromatography application.

We report preliminary testing of "GeneTrack", an instrument designed for the specific application of multiplexed short tandem repeat (STR) DNA analysis. The system supports a glass microdevice with 16 lanes of 20 cm effective length and double-T cross injectors. A high-speed galvanometer-scanned four-color detector was specially designed to accommodate the high elution rates on the microdevice. All aspects of the system were carefully matched to practical crime lab requirements for rapid reproducible analysis of crime-scene DNA evidence in conjunction with the United States DNA database (CODIS). Statistically significant studies demonstrate that an absolute, three-sigma, peak accuracy of 0.4-0.9 base pair (bp) can be achieved for the CODIS 13-locus multiplex, utilizing a single channel per sample. Only 0.5 microL of PCR product is needed per lane, a significant reduction in the consumption of costly chemicals in comparison to commercial capillary machines. The instrument is also designed to address problems in temperature-dependent decalibration and environmental sensitivity, which are weaknesses of the commercial capillary machines for the forensics application.

A high-performance multilane microdevice system designed for the DNA forensics laboratory.

We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.

Microdevice DNA forensics by the simple tandem repeat method.

Functional high-density micro-arrays for mass spectrometry enable rapid picolitre-volume aliquoting and ultrasensitive analysis of microscale samples, for example, single cells.

High-density micro-arrays for mass spectrometry.

A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.

Article	Year
Evaporation driven pumping for chromatography application. Lab on a chip\| PubMed ID: 15100814	2002
A high-performance multilane microdevice system designed for the DNA forensics laboratory. Electrophoresis\| PubMed ID: 15188257	2004
Microdevice DNA forensics by the simple tandem repeat method. Journal of chromatography. A\| PubMed ID: 16569579	2006
High-density micro-arrays for mass spectrometry. Lab on a chip\| PubMed ID: 20938499	2010
Connecting μ-fluidics to electron microscopy. Journal of structural biology\| PubMed ID: 22094535	2012

Nils Goedecke

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