a-method-for-lineage-tracing-corneal-cells-using-multi-color

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Lineage tracing experiments define the origin, fate and behavior of cells in a specific tissue or organism. This technique has been successfully applied ...

Israel Institute of Technology

The purpose of this review is to highlight the recent literature and emerging data describing clinical situations in which menstrual suppression may improve symptoms and quality of life for adolescents. A variety of conditions occurring frequently in adolescents and young adults, including heavy menstrual bleeding, and dysmenorrhea as well as gynecologic conditions such as endometriosis and pelvic pain, can safely be improved or alleviated with appropriate menstrual management.

Menstrual suppression for adolescents.

Accumulating evidence supports the dogma that the corneal epithelium is regenerated by stem cells located exclusively in the limbal niche, at the corneal periphery. Accordingly, limbal stem cells (LSCs) give rise to progenitors that proliferate and migrate centripetally to repopulate the corneal epithelium, which has a short turnover. Moreover, LSC loss leads to corneal opacity and blindness, while limbal grafting restores patients' vision. However, contradicting data suggested that the limbus does not participate in corneal homeostasis and that the cornea contains stem cells. As of today, only indirect evidence for limbal cell migration under homeostasis or injury has been demonstrated. Here, we performed lineage tracing experiments using R26R-Confetti mice to follow K14+ limbal/corneal epithelial cells stochastically induced to express one out of four fluorescent genes. In homeostasis, radial limbal stripes of slow migrating cells proceeded toward the corneal center while, infrequently, slow cycling limbal clones resembling quiescent stem cells were observed. Additionally, rare corneal clones that did not migrate centripetally, but survived for over 4 months, were inspected. In contrast to limbal stripes, corneal clusters had minor contribution to tissue replenishment in homeostasis. Corneal cells, however, significantly contributed to mild wound repair while large limbal streaks appeared within a week following severe wounding that coincided with partial loss of corneal transparency. This data suggest that the mouse limbus largely contributes to corneal renewal while corneal progenitor cells have a long turnover and, therefore, may be able to maintain the corneal epithelium for several months.

Lineage tracing of stem and progenitor cells of the murine corneal epithelium.

To explore the use of social media for recruitment of adolescents and young adults in the United States and to describe how they learn and feel about abortion.

Exploring abortion attitudes of US adolescents and young adults using social media.

The purpose of this review is to revisit the inception of the WHO's medical eligibility criteria for contraceptive use (MEC), particularly its objectives and methodology, and to describe its impact over the last 20 years in the field of family planning. New recommendations are summarized from the newly released fifth edition of the guidance.

Article	Year
Menstrual suppression for adolescents. Current opinion in obstetrics & gynecology\| PubMed ID: 25110977	2014
Lineage tracing of stem and progenitor cells of the murine corneal epithelium. Stem cells (Dayton, Ohio)\| PubMed ID: 25187087	2015
Exploring abortion attitudes of US adolescents and young adults using social media. Contraception\| PubMed ID: 25537853	2015
The WHO's medical eligibility criteria for contraceptive use: 20 years of global guidance. Current opinion in obstetrics & gynecology\| PubMed ID: 26390246	2015

Anna Altshuler

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