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A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

DOI:
10.3791/53370-v

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07:48 min

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December 18, 2015

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, , , , , , ,
1Department of Genetics and Developmental Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion,Israel Institute of Technology, 2Department of Ophthalmology,Hillel Yaffe Medical Center, 3Bioimging Center, Biomedical Core Facility, The Ruth and Bruce Rappaport Faculty of Medicine, Technion,Israel Institute of Technology

Chapters

  • 00:05Title
  • 00:42Crossing Transgenic Lines of Interest and Tamoxifen Induction
  • 02:19Combining Corneal Injury with Tamoxifen Induction
  • 03:18Preparing Tissue for Imaging
  • 05:19Confocal Microscopy and Image Analysis
  • 06:04Results: Corneal Lineage Tracing under Homeostasis and Injury
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.

Tags

Corneal CellsLineage TracingMulti-color Fluorescent Reporter MiceR26R-Confetti MouseDevelopmental BiologyStem Cell BiologyFluorescent MicroscopyOcular SurfaceCorneal Epithelium

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