Miguel Alcalde

Department of Biocatalysis, Institute of Catalysis, CSIC

Affiliated withInstitute of Catalysis, CSIC

Research Area

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 10	2016

Other Publications

Article	Year
Modification of galactose oxidase to introduce glucose 6-oxidase activity. Chembiochem : a European journal of chemical biology\| PubMed ID: 12203977	2002
Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Applied and environmental microbiology\| PubMed ID: 12571021	2003
Screening mutant libraries in Saccharomyces cerevisiae. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 12824572	2003
Colorimetric assays for screening laccases. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 12824583	2003
Preparing libraries in Saccharomyces cerevisiae. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 12824597	2003
Colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases. Journal of biomolecular screening\| PubMed ID: 14599353	2002
Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3. Journal of biomolecular screening\| PubMed ID: 15006137	2004
Effect of carbohydrate fatty acid esters on Streptococcus sobrinus and glucosyltransferase activity. Carbohydrate research\| PubMed ID: 15063188	2004
Use and improvement of microbial redox enzymes for environmental purposes. Microbial cell factories\| PubMed ID: 15287990	2004
Immobilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F on Eupergit C supports. Biotechnology progress\| PubMed ID: 15458325	2004
Screening mutant libraries of fungal laccases in the presence of organic solvents. Journal of biomolecular screening\| PubMed ID: 16103414	2005
Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation. Angewandte Chemie (International ed. in English)\| PubMed ID: 16254934	2005
Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299. Journal of biotechnology\| PubMed ID: 16513200	2006
Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides. Journal of agricultural and food chemistry\| PubMed ID: 16608216	2006
Environmental biocatalysis: from remediation with enzymes to novel green processes. Trends in biotechnology\| PubMed ID: 16647150	2006
Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships. The Journal of biological chemistry\| PubMed ID: 16740638	2006
Combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases. Combinatorial chemistry & high throughput screening\| PubMed ID: 17168677	2006
Transformation of polycyclic aromatic hydrocarbons by laccase is strongly enhanced by phenolic compounds present in soil. Environmental science & technology\| PubMed ID: 17533865	2007
In vitro evolution of a fungal laccase in high concentrations of organic cosolvents. Chemistry & biology\| PubMed ID: 17884637	2007
Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra. Proteins\| PubMed ID: 17932916	2008
Engineering and Applications of fungal laccases for organic synthesis. Microbial cell factories\| PubMed ID: 19019256	2008
Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide. Combinatorial chemistry & high throughput screening\| PubMed ID: 19075602	2008
Laccases and their applications: a patent review. Recent patents on biotechnology\| PubMed ID: 19075849	2008
Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast. Microbial cell factories\| PubMed ID: 20298573	2010
Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 20676972	2010
Laboratory evolution of high-redox potential laccases. Chemistry & biology\| PubMed ID: 20851352	2010
Screening β-fructofuranosidases mutant libraries to enhance the transglycosylation rates of β-(2→6) fructooligosaccharides. Combinatorial chemistry & high throughput screening\| PubMed ID: 21599626	2011
Directed evolution of fungal laccases. Current genomics\| PubMed ID: 21966249	2011
Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase. The Biochemical journal\| PubMed ID: 21980920	2012
Risk of recurrence of non-metastatic breast cancer in women under 40 years: a population-registry cancer study in a European country. The breast journal\| PubMed ID: 22211922	2012
Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports. Advanced materials (Deerfield Beach, Fla.)\| PubMed ID: 22299142	2011
Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes. Bioengineered bugs\| PubMed ID: 22572788	2012
Development of chimeric laccases by directed evolution. Biotechnology and bioengineering\| PubMed ID: 22729887	2012
Covalent immobilization of cyclodextrin glucosyltransferase (CGTase) in activated silica and Sepharose. Indian journal of biochemistry & biophysics\| PubMed ID: 22908412	2002
Blood tolerant laccase by directed evolution. Chemistry & biology\| PubMed ID: 23438751	2013
Widening the pH activity profile of a fungal laccase by directed evolution. Chembiochem : a European journal of chemical biology\| PubMed ID: 23592228	2013
Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production. Microbial cell factories\| PubMed ID: 23617537	2013
Functional expression of a blood tolerant laccase in Pichia pastoris. BMC biotechnology\| PubMed ID: 23627343	2013
New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass. BMC biotechnology\| PubMed ID: 24159930	2013
Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution. PloS one\| PubMed ID: 24614282	2014
Directed evolution of unspecific peroxygenase from Agrocybe aegerita. Applied and environmental microbiology\| PubMed ID: 24682297	2014
Bioelectrochemical oxidation of water. Journal of the American Chemical Society\| PubMed ID: 24725275	2014
Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae. Bioengineered\| PubMed ID: 24830983	2014
Self-powered wireless carbohydrate/oxygen sensitive biodevice based on radio signal transmission. PloS one\| PubMed ID: 25310190	2014
Laccase engineering: from rational design to directed evolution. Biotechnology advances\| PubMed ID: 25545886	2015
Engineering the ligninolytic enzyme consortium. Trends in biotechnology\| PubMed ID: 25600621	2015
Tandem-yeast expression system for engineering and producing unspecific peroxygenase. Enzyme and microbial technology\| PubMed ID: 26002501	2015
Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides. Applied and environmental microbiology\| PubMed ID: 26162870	2015
Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol. Angewandte Chemie (International ed. in English)\| PubMed ID: 26607550	2016
Synthesis of 1-Naphthol by a Natural Peroxygenase Engineered by Directed Evolution. Chembiochem : a European journal of chemical biology\| PubMed ID: 26677801	2016