10,888 Views
•
10:50 min
April 01, 2016
DOI:
10.3791/53761-v
We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.
07:18
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
Related Videos
10703 Views
11:39
Mating-based Overexpression Library Screening in Yeast
7679 Views
07:55
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
7996 Views
09:01
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
30500 Views
08:57
High-throughput Yeast Plasmid Overexpression Screen
16365 Views
11:59
Competitive Genomic Screens of Barcoded Yeast Libraries
18271 Views
05:49
Protein Engineering by Yeast Surface Display
1187 Views
12:13
Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
10340 Views
10:57
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
9753 Views
13:22
Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
1817 Views
Read Article
Cite this Article
Viña-Gonzalez, J., Gonzalez-Perez, D., Alcalde, M. Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening. J. Vis. Exp. (110), e53761, doi:10.3791/53761 (2016).
Download .ris file
Copy
Share Video
.