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Directed Evolution Method in Saccharomyces cerevisiae

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Article DOI: 10.3791/53761 10:50 min April 1st, 2016
April 1st, 2016



We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.



Directed Evolution Method Saccharomyces Cerevisiae Mutant Library Screening Laboratory Creation Focused Directed Evolution Experiments Assembly And Cloning Simplicity Robustness Fungal Aryl-alcohol Oxidase AAO Random Mutagenesis Recombination M1 Region M2 Region Mutagenic PCR In Vivo Splicing Yeast
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