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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

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Biology

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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

DOI:

10.3791/53761-v

•

10:50 min

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April 01, 2016

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Javier Viña-Gonzalez, David Gonzalez-Perez, Miguel Alcalde

¹Department of Biocatalysis,Institute of Catalysis, CSIC

Chapters

00:05Title
00:42Mutant Library Construction
05:46High-Throughput Screening Assay
07:54Results: Successful Construction and Screening of an AAO Mutant Library
09:42Conclusion

Summary

Automatic Translation

We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.

Tags

Directed Evolution Saccharomyces Cerevisiae Mutant Library Aryl-alcohol Oxidase (AAO)Random Mutagenesis PCR In Vivo Splicing Overlapping Regions High-fidelity PCR Screening

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