mimicking-function-signaling-proteins-toward-artificial-signal

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Signal transduction pathways, which control the response of cells to various environmental signals, are mediated by the function of signaling proteins ...

Weizmann Institute of Science

The design and function of a synthetic "chemical transducer" that can generate an unnatural communication channel between two proteins is described. Specifically, we show how this transducer enables platelet-derived growth factor to trigger (in vitro) the catalytic activity of glutathione-s-transferase (GST), which is not its natural enzyme partner. GST activity can be further controlled by adding specific oligonucleotides that switch the enzymatic reaction on and off. We also demonstrate that a molecular machine, which can regulate the function of an enzyme, could be used to change the way a prodrug is activated in a "programmable" manner.

Protein-Protein Communication and Enzyme Activation Mediated by a Synthetic Chemical Transducer.

Artificial signal transduction therapy: a futuristic approach to disease treatment.

Pancreatic lipase (triacylglycerol lipase EC 3.1.1.3) is an essential enzyme in hydrolysis of dietary fat. Dietary fat, especially polyunsaturated fatty acids (PUFA), regulate pancreatic lipase (PNLIP); however, the molecular mechanism underlying this regulation is mostly unknown. As PUFA are known to regulate expression of proliferator-activated receptor gamma (PPARγ), and as we identified in-silico putative PPARγ binding sites within the putative PNLIP promoter sequence, we hypothesized that PUFA regulation of PNLIP might be mediated by PPARγ.

Article	Year
Protein-Protein Communication and Enzyme Activation Mediated by a Synthetic Chemical Transducer. Journal of the American Chemical Society\| PubMed ID: 25955617	2015
Artificial signal transduction therapy: a futuristic approach to disease treatment. Future medicinal chemistry\| PubMed ID: 26510968	2015
PPARγ regulates exocrine pancreas lipase. Biochimica et biophysica acta\| PubMed ID: 27663184	2016

Ronny Peri-Naor

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