an-optimized-protocol-for-efficient-radiolabeling-gold-nanoparticles

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a <sup>125</sup>I-labeled Azide Prosthetic Group

Here, we demonstrate a detailed protocol for the radiosynthesis of a 125I-labeled azide prosthetic group and its application to the efficient ...

an-efficient-method-for-selective-desalination-radioactive-iodine

An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter

Here, we demonstrate a detail protocol for the preparation of nanomaterials-embedded composite membranes and its application to the efficient and ...

Korea Atomic Energy Research Institute

Kyungpook National University

Herein we report an efficient method for iodine radioisotope labeling of cyclooctyne-containing molecules using copper-free click reaction. For this study, radioiodination using the tin precursor 2 was carried out at room temperature to give (125)I-labeled azide ([(125)I]1) with high radiochemical yield (85%) and excellent radiochemical purity. Dibenzocyclooctyne (DBCO) containing cRGD peptide and gold nanoparticle were labeled with [(125)I]1 at 37°C for 30min to give triazoles with good radiochemical yields (67-95%). We next carried out tissue biodistribution study of [(125)I]1 in normal ICR mice to investigate the level of organ accumulation which needs to be considered for pre-targeted in vivo imaging. Large amount of [(125)I]1 distributed rapidly in liver and kidney from bloodstream and underwent rapid renal and hepatobiliary clearance. Moreover [(125)I]1 was found to be highly stable (>92%) in mouse serum for 24h. Therefore [(125)I]1 could be used as a potentially useful radiotracer for pre-targeted imaging. Those results clearly indicated that the present radiolabeling method using copper free click reaction would be quite useful for both in vitro and in vivo labeling of DBCO group containing molecules with iodine radioisotopes.

Efficient method for iodine radioisotope labeling of cyclooctyne-containing molecules using strain-promoted copper-free click reaction.

Herein we report the radiosynthesis of a pyridine derived azide prosthetic group for iodine radioisotope labeling of dibenzocyclooctyne (DBCO) conjugated molecules. The radiolabeling of the stannylated precursor 2 was conducted using [(125)I]NaI and chloramine-T to give (125)I-labeled azide ([(125)I]1) with high radiochemical yield (72±8%, n=4) and radiochemical purity (>99%). Using (125)I-labeled azide ([(125)I]1), cyclic RGD peptide and near infrared fluorescent molecule were efficiently labeled with modest to good radiochemical yields. The biodistribution study and SPECT/CT images showed that [(125)I]1 underwent rapid renal clearance. These results clearly demonstrated that [(125)I]1 could be used as an useful radiotracer for in vivo pre-targeted imaging as well as efficient in vitro radiolabeling of DBCO containing molecules.

Synthesis and evaluation of an (125)I-labeled azide prosthetic group for efficient and bioorthogonal radiolabeling of cyclooctyne-group containing molecules using copper-free click reaction.

In this report, we present a rapid and highly efficient method for radioactive iodine labeling of trans-cyclooctene group conjugated biomolecules using inverse-electron-demand Diels-Alder reaction. Radioiodination reaction of the tetrazine structure was carried out using the stannylated precursor 2 to give (125)I-labeled azide ([(125)I]1) with high radiochemical yield (65±8%) and radiochemical purity (>99%). For radiolabeling application of [(125)I]1, trans-cyclooctene derived cRGD peptide and human serum albumin were prepared. These substrated were reacted with [(125)I]1 under mild condition to provide the radiolabeled products [(125)I]6 and [(125)I]8, respectively, with excellent radiochemical yields. The biodistribution study of [(125)I]8 in normal ICR mice showed significantly lower thyroid uptake values than that of (125)I-labeled human serum albumin prepared by a traditional radiolabeling method. Therefore [(125)I]8 will be a useful radiolabeled tracer in various molecular imaging and biological studies. Those results clearly demonstrate that [(125)I]1 will be used as a valuable prosthetic group for radiolabeling of biomolecules.

Highly efficient method for (125)I-radiolabeling of biomolecules using inverse-electron-demand Diels-Alder reaction.

There has been worldwide attention on the efficient removal of radioactive iodine, because it is commonly released in nuclear plant accidents. Increasing concerns on environmental problems due to the radioactive iodine are leading us to develop stable and sustainable technology for remediation of radioelement contaminants. In this work, we report a highly efficient chromatographic method for specific and rapid capture of radioactive iodine. The gold nanoparticles immobilized dextran gel columns showed excellent removal capabilities of radioactive iodine in various conditions. These results suggested that our platform technology can be a promising method for the desalination of radioactive iodines in water.

Gold-Nanoparticle-Immobilized Desalting Columns for Highly Efficient and Specific Removal of Radioactive Iodine in Aqueous Media.

We herein report a new bioremediation method using a radiation-resistant bacterium. Biogenic gold nanomaterial-containing Deinococcus radiodurans R1 showed excellent capability for the removal of radioactive iodine (>99%) in several aqueous solutions. These observations demonstrated that our remediation system would be efficiently applied to the treatment of radioactive wastes.

Efficient bioremediation of radioactive iodine using biogenic gold nanomaterial-containing radiation-resistant bacterium, Deinococcus radiodurans R1.

To assess the risk posed by a toxic chemical to human health, it is essential to quantify its uptake in a living subject. This study aims to investigate the biological distribution of inhaled polyhexamethylene guanidine (PHMG) aerosol particle, which is known to cause severe pulmonary damage. By labeling with indium-111 (In), we quantified the uptake of PHMG for up to 7 days after inhalation exposure in rats. The data demonstrate that PHMG is only slowly cleared, with approximately 74% of inhaled particles persisting in the lungs after 168 h. Approximately 5.3% of inhaled particles were also translocated to the liver after 168 h, although the level of redistribution to other tissues, including the kidneys and spleen, was minimal. These observations suggest that large uptake and slow clearance may underlie the fatal inhalation toxicity of PHMG in humans.

ArticleTotal : 2	Year
Publication title Cited by 5	2016
Publication title Cited by 3	2018

Article	Year
Efficient method for iodine radioisotope labeling of cyclooctyne-containing molecules using strain-promoted copper-free click reaction. Bioorganic & medicinal chemistry\| PubMed ID: 25960325	2015
Synthesis and evaluation of an (125)I-labeled azide prosthetic group for efficient and bioorthogonal radiolabeling of cyclooctyne-group containing molecules using copper-free click reaction. Bioorganic & medicinal chemistry letters\| PubMed ID: 26748695	2016
Highly efficient method for (125)I-radiolabeling of biomolecules using inverse-electron-demand Diels-Alder reaction. Bioorganic & medicinal chemistry\| PubMed ID: 27134118	2016
Gold-Nanoparticle-Immobilized Desalting Columns for Highly Efficient and Specific Removal of Radioactive Iodine in Aqueous Media. ACS applied materials & interfaces\| PubMed ID: 27758102	2016
Efficient bioremediation of radioactive iodine using biogenic gold nanomaterial-containing radiation-resistant bacterium, Deinococcus radiodurans R1. Chemical communications (Cambridge, England)\| PubMed ID: 28317956	2017
Quantification of inhaled aerosol particles composed of toxic household disinfectant using radioanalytical method. Chemosphere\| PubMed ID: 29852464	2018

Ha Eun Shim

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