from-natural-product-to-its-biosynthetic-gene-cluster-demonstration

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from <em>Streptomyces diastatochromogenes</em> T&#252;6028

Streptomyces strains are known for their capability to produce a lot of different compounds with various bioactivities. Cultivation under different ...

Albert-Ludwigs-Universität Freiburg, Germany

Two new amides, named N-acetyl-2,4,10,17-tetrahydroxyheptadecylamine (1) and N-acetyl-3,5,11,18-tetrahydroxyoctadecyl-2-amine (2), were isolated from a halotolerant fungus, Myrothecium sp. GS-17. Their structures were identified on the basis of spectroscopic characteristics. The cancer cell cytotoxicities of two compounds were evaluated, and compound 2 exhibited weak cytotoxicity in HL-60 cell line.

Two new amides from a halotolerant fungus, Myrothecium sp. GS-17.

Recently we described an unusual way of activating a cryptic gene cluster when we explored the origin of the bald phenotype of Streptomyces calvus. Complementation of S. calvus with a correct copy of bldA restored sporulation and additionally promoted production of a new natural products. In this study we report on the expression of bldA in several Streptomyces strains that have been described as "poorly sporulating" strains. In seven out of 15 cases, HPLC profiling revealed the production of new compounds, and in two cases the overproduction of known compounds. Two compounds were isolated and their structures were determined.

Changing Biosynthetic Profiles by Expressing bldA in Streptomyces Strains.

A new polyketide, penicillolide (1) was isolated from the fermentation broth of the marine-derived fungus Penicillium sacculum GT-308. Compound 1 is a polyketide with a unique carbon skeleton. The structure of this compound was established via extensive spectroscopic analyses including 1D-, 2D-NMR, and HRESI-MS.

Article	Year
Two new amides from a halotolerant fungus, Myrothecium sp. GS-17. The Journal of antibiotics\| PubMed ID: 25269461	2015
Changing Biosynthetic Profiles by Expressing bldA in Streptomyces Strains. Chembiochem : a European journal of chemical biology\| PubMed ID: 26255983	2015
A new polyketide, penicillolide from the marine-derived fungus Penicillium sacculum. Natural product research\| PubMed ID: 26499896	2016

Songya Zhang

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