Journal
/
Genetics
/
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

DOI:
10.3791/54952-v

•

09:08 min

•

January 13, 2017

•

, , ,
1Department of Pharmaceutical Biology and Biotechnology,Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology,Universität Siegen

Chapters

  • 00:05Title
  • 00:54Identification of a Natural Product with Antibiotic Property
  • 03:54Propose Biosynthetic Model of the New Isolated Compound
  • 05:02Identification of the Biosynthetic Gene Cluster
  • 06:34Verification of the Biosynthetic Gene Cluster
  • 07:40Results: HPLC Analysis of S. diastatochromogenes with Inactivated pokPI Gene
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.

Tags

Natural ProductBiosynthetic Gene ClusterPolyketomycinStreptomyces DiastatochromogenesAntibiotic ActivityGenome MiningStreptomycesAntibiotic ProducerBiosynthetic PathwayCompound ExtractionHPLCDisc Diffusion AssayTest StrainAntibiotic

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Read Article