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JoVE Journal
Genetics

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From a Natural Product to Its Biosynthetic Gene Cluster
 

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Article doi: 10.3791/54952
January 13th, 2017

Summary January 13th, 2017

Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.

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