time-dependent-increase-network-response-to-stimulation-neuronal-cell

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Micro-electrode arrays (MEAs) can be used to investigate drug toxicity, design paradigms for next-generation personalized medicine, and study network ...

George Mason University

Lofexidine for opioid detoxification.

In vitro neuronal networks cultured on microelectrode arrays enable the study of network electrophysiology on a fundamental level. Neuronal response to electrical stimulation is an area of interest at the laboratory bench and in the clinic, given its wide application for remedying neurological disorders. Here we investigated the change in cortical network response over time to varied amounts of charge used for stimulation, which may lead to a phenomenon known as selective adaptation. There is a charge threshold that invokes a reverberating network response; when stimulating at 900 mV, five stimulation electrodes were required to elicit a response across the entire network. Stimulating with more charge leads to greater synaptic depression over time when constant periodic stimulation is applied. Stimulating with 5 electrodes led to a decrease in network response to stimulation, whereas stimulating with 12 electrodes led to an extinction of network response. The previously hypothesized selective adaptation mechanism was not observed, implying that our random cortical assemblies have homogeneous excitatory and inhibitory subnetworks.

Article	Year
Lofexidine for opioid detoxification. Psychiatry (Edgmont (Pa. : Township))\| PubMed ID: 21179638	2005
Synaptic depression depends on charge delivered to network. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference\| PubMed ID: 28268679	2016

Sharon Jose

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