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Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

DOI:
10.3791/55726-v

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10:45 min

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May 29, 2017

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, , ,
1Krasnow Institute for Advanced Study,George Mason University, 2Neural Engineering, Bioengineering,George Mason University, 3Neural Engineering, Computer Science,George Mason University, 4Electrical and Computer Engineering,George Mason University

Chapters

  • 00:05Title
  • 01:04Embryo Removal and Brain Extraction
  • 03:19Frontal Cortext Removal
  • 04:46Cell Dissociation
  • 06:48Plating Cells and Maintaining the Cultures
  • 08:01Visual Inspections and Recording
  • 09:24Results: Trained Networks Have Significantly Altered Spike Frequencies
  • 10:19Conclusion

Summary

Automatic Translation

Automatic Translation

Mouse neuronal cells cultured on multi-electrode arrays display an increase in response following electrical stimulation. This protocol demonstrates how to culture neurons, how to record activity, and how to establish a protocol to train the networks to respond to patterns of stimulation.

Tags

Keywords: Microelectrode ArraysNeuronal Cell CulturesNetwork DynamicsLearning And MemoryDrug ToxicityPersonalized MedicineDissectionDissociationLamininL-15 Medium

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