cavernous-nerve-stimulation-recording-intracavernous-pressure

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for ...

University of Vermont

Transurethral intraprostatic ethanol chemoablation of the prostate has shown promising preliminary clinical results for benign prostatic hyperplasia with some variability in clinical outcome. This is likely due to the uneven prostate diffusion caused by varying resistance of the tissue type in which the tip of the needle is embedded. We examined whether the distribution of the injectable in the canine prostate could be improved using a microporous hollow fiber catheter (Twin Star Medical, Minneapolis, Minnesota).

Comparison of intraprostatic ethanol diffusion using a microporous hollow fiber catheter versus a standard needle.

We examined the effect of caffeine (Sigma®) on voiding patterns in mice and characterized potential changes in bladder function and sensory signaling.

Caffeine ingestion causes detrusor overactivity and afferent nerve excitation in mice.

To develop an economic, practical and readily available animal model for preclinical testing of urethral bulking therapies, as well as to establish feasible experimental methods that allow for complete analysis of hard microparticle bulking agents.

Rat animal model for preclinical testing of microparticle urethral bulking agents.

Objectives. To test the physical properties and host response to the bioceramic particles, silica-calcium phosphate (SCPC10) and Cristobalite, in a rat animal model and compare their biocompatibility to the current clinically utilized urethral bulking materials. Material and Methods. The novel bulking materials, SCPC10 and Cristobalite, were suspended in hyaluronic acid sodium salt and injected into the mid urethra of a rat. Additional animals were injected with bulking materials currently in clinical use. Physiological response was assessed using voiding trials, and host tissue response was evaluated using hard tissue histology and immunohistochemical analysis. Distant organs were evaluated for the presence of particles or their components. Results. Histological analysis of the urethral tissue five months after injection showed that both SCPC10 and Cristobalite induced a more robust fibroblastic and histiocytic reaction, promoting integration and encapsulation of the particle aggregates, leading to a larger bulking effect. Concentrations of Ca, Na, Si, and P ions in the experimental groups were comparable to control animals. Conclusions. This side-by-side examination of urethral bulking agents using a rat animal model and hard tissue histology techniques compared two newly developed bioactive ceramic particles to three of the currently used bulking agents. The local host tissue response and bulking effects of bioceramic particles were superior while also possessing a comparable safety profile.

Article	Year
Comparison of intraprostatic ethanol diffusion using a microporous hollow fiber catheter versus a standard needle. The Journal of urology\| PubMed ID: 22425049	2012
Caffeine ingestion causes detrusor overactivity and afferent nerve excitation in mice. The Journal of urology\| PubMed ID: 22999550	2012
Rat animal model for preclinical testing of microparticle urethral bulking agents. International journal of urology : official journal of the Japanese Urological Association\| PubMed ID: 25581400	2015
Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model. Advances in urology\| PubMed ID: 27688751	2016

Travis Mann-Gow

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