radionuclide-fluorescence-reporter-gene-imaging-to-track-tumor

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Metastasis is responsible for most cancer deaths. Despite extensive research, the mechanistic understanding of the complex processes governing metastasis ...

King's College London

Accumulation of iodide and other substrates via the human sodium/iodide symporter (hNIS) is fundamental to imaging and therapy of thyroid disease, hNIS reporter gene imaging and hNIS-mediated gene therapy. There is no readily available positron emission tomography (PET) tracer for hNIS. Our aim was to develop a colon carcinoma cell line stably expressing hNIS, and use it to evaluate a novel hNIS PET tracer, [18F]-tetrafluoroborate.

Evaluation of [18F]-tetrafluoroborate as a potential PET imaging agent for the human sodium/iodide symporter in a new colon carcinoma cell line, HCT116, expressing hNIS.

Accumulation d&#39;iodure et autres substrats via le symport humaine/iodure de sodium (hNIS) est fondamentale pour l&#39;imagerie et de th&eacute;rapie de la maladie thyro&iuml;dienne, l&#39;hNIS journaliste g&egrave;ne imagerie et th&eacute;rapie g&eacute;nique m&eacute;di&eacute;e par hNIS. Il n&#39;y a aucun traceur de tomographie par &eacute;mission de positron facilement disponibles (PET) pour hNIS. Notre objectif &eacute;tait de d&eacute;velopper une lign&eacute;e de cellules de carcinome du c&ocirc;lon exprimant de mani&egrave;re stable hNIS et utilisez-le pour &eacute;valuer un traceur roman hNIS PET, [18F]-t&eacute;trafluoroborate.

&Eacute;valuation du [18F]-t&eacute;trafluoroborate comme agent d&#39;imagerie PET potentiel pour le symport humaine/iodure de sodium dans une lign&eacute;e de cellules de carcinome du c&ocirc;lon nouvelle, HCT116, exprimant hNIS.

L&#39;accumulo di ioduro e altri substrati attraverso la symporter sodio/Ioduro umano (hNIS) &egrave; fondamentale per l&#39;imaging e la terapia delle malattie della tiroide, hNIS reporter gene imaging e terapia genica mediata da hNIS. Non non c&#39;&egrave; nessun elemento tracciante prontamente disponibili tomografia ad emissione di positroni (PET) per hNIS. Il nostro obiettivo era quello di sviluppare una linea cellulare di carcinoma del colon che esprimono stabilmente hNIS e utilizzarlo per valutare un tracciante hNIS romanzo PET, [18F]-Tetrafluoborato.

Valutazione di [18F]-Tetrafluoborato come un potenziale agente di imaging PET per il symporter sodio/Ioduro umano in una nuova linea cellulare del colon carcinoma, HCT116, esprimendo hNIS.

Nitrogen-13 has a 10-min half-life which places time constraints on the complexity of viable synthetic methods for its incorporation into PET imaging agents. In exploring ways to overcome this limitation, we have used the Ugi reaction to develop a rapid one-pot method for radiolabelling peptidic molecules using [N]NHas a synthetic precursor.

Article	Year
Evaluation of [18F]-tetrafluoroborate as a potential PET imaging agent for the human sodium/iodide symporter in a new colon carcinoma cell line, HCT116, expressing hNIS. Nuclear medicine communications\| PubMed ID: 21085047	2011
Convergent synthesis ofN-labelled Peptidic structures using aqueous [N]NH. EJNMMI radiopharmacy and chemistry\| PubMed ID: 29503857	2017

Julia Blower

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