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Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

DOI:
10.3791/57088-v

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10:04 min

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March 13, 2018

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, , , , , ,
1Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences,King’s College London

Chapters

  • 00:04Title
  • 01:40Engineering and Characterization of Cancer Cells to Express the Radionuclide-fluorescence Fusion Reporter NIS-FP and [18F]BF4 &#45 Synthesis
  • 04:04In Vivo Imaging of NIS-FP Expressing Cells by nanoPET/CT
  • 05:40Ex Vivo Analyses
  • 06:33Results: Radiotracer Uptake in Cell Lines is Due to Specific NIS-FP Expression
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for preclinical in vivo tracking of cancer metastasis. It is based on a radionuclide-fluorescence reporter combining the sodium iodide symporter, detected by non-invasive [18F]tetrafluoroborate-PET, and a fluorescent protein for streamlined ex vivo confirmation. The method is applicable for preclinical in vivo cell tracking beyond tumor biology.

Tags

Keywords: RadionuclideFluorescenceReporter GeneTumor ProgressionRodent Tumor ModelsPETIn Vivo ImagingCell TrackingAutomated SynthesisPreclinical TestingCancer BiologyTherapeutic Development

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