crispr-mediated-reorganization-of-chromatin-loop-structure

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Recent studies have clearly shown that long-range, three-dimensional chromatin looping interactions play a significant role in the regulation of gene ...

Stanford University School of Medicine

It is increasingly evident that long noncoding RNAs may play the roles of both oncogenes and tumor suppressors during cancer development. A new study from Piipponen et al. provides evidence that a long noncoding RNA, PICSAR, promotes cutaneous squamous cell carcinoma development through activation of extracellular signal-regulated kinase signaling. Because specific inhibition of PICSAR suppresses tumor growth, this long noncoding RNA may serve as a useful diagnostic marker and therapeutic target for cutaneous squamous cell carcinoma.

PICSAR: Long Noncoding RNA in Cutaneous Squamous Cell Carcinoma.

Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

Article	Year
PICSAR: Long Noncoding RNA in Cutaneous Squamous Cell Carcinoma. The Journal of investigative dermatology\| PubMed ID: 27450499	2016
Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping. Nature communications\| PubMed ID: 28703221	2017

Yunhai Luo

Biography

JoVE Journal Publications

Other Publications