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1Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, 2Program in Cancer Biology,Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, 4Department of Biology,Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
Chapters
- 00:04Title
- 01:02Plasmid Preparation and gRNA Insertion
- 03:26Lentivirus Production
- 05:12Lentivirus Transduction of Cells / Cell Dimerization
- 07:08Results: CLOuD9 Induces Reversible β-Globin Promoter-LCR Looping
- 08:55Conclusion
Chromatin looping plays a significant role in gene regulation; however, there have been no technological advances that allow for selective and reversible modification of chromatin loops. Here we describe a powerful system for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9), demonstrated to selectively and reversibly modulate gene expression at targeted loci.
