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JoVE Journal
Genetics

This content is Open Access.

CRISPR-Mediated Reorganization of Chromatin Loop Structure
 

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Article DOI: 10.3791/57457 09:20 min September 14th, 2018
September 14th, 2018

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Summary

Chromatin looping plays a significant role in gene regulation; however, there have been no technological advances that allow for selective and reversible modification of chromatin loops. Here we describe a powerful system for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9), demonstrated to selectively and reversibly modulate gene expression at targeted loci.

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Tags

CRISPR-mediated Chromatin Loop Structure Gene Expression Chromatin Organization Transcriptional Dynamics Alter Chromosomal Architecture Long-range Chromatin Looping Reverse Chromatin Context Non-toxic Dimerizer Orthogonal Species Of Dcas9 Wide Applicability CRISPR-guide RNA Sequences Cell Cultures Plasmid Maps Lentiviral CRISPR Plasmid BsmB1 Alkaline Phosphatase Digestion Buffer DTT
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