Michael C. Jewett

Department of Chemical and Biological Engineering, Northwestern University

Affiliated withNorthwestern University Northwestern University

Research Area

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 71	2019

Other Publications

Article	Year
Update on designing and building minimal cells. Current opinion in biotechnology\| PubMed ID: 20638265	2010
Biology by design: from top to bottom and back. Journal of biomedicine & biotechnology\| PubMed ID: 21052559	2010
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science (New York, N.Y.)\| PubMed ID: 21764749	2011
Cell-free synthetic biology: thinking outside the cell. Metabolic engineering\| PubMed ID: 21946161	2012
Cell-free protein synthesis: Applications come of age. Biotechnology advances\| PubMed ID: 22008973	2011
Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry. Current opinion in biotechnology\| PubMed ID: 22483202	2012
Synthetic in vitro circuits. Current opinion in chemical biology\| PubMed ID: 22676890	2012
Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis. ACS synthetic biology\| PubMed ID: 22737598	2012
In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation. Molecular systems biology\| PubMed ID: 23799452	2013
Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis. Biotechnology and bioengineering\| PubMed ID: 23832321	2013
Mapping Condition Dependent Regulation of Lipid Metabolism in Saccharomyces cerevisiae. G3 (Bethesda, Md.)\| PubMed ID: 24062529	2013
Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology journal\| PubMed ID: 24323955	2014
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation. ACS synthetic biology\| PubMed ID: 24328168	2014
A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe. Biotechnology journal\| PubMed ID: 24677809	2014
Quantifying position-dependent codon usage bias. Molecular biology and evolution\| PubMed ID: 24710515	2014
The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction. Nucleic acids research\| PubMed ID: 24792158	2014
Cell-free synthetic biology special issue. ACS synthetic biology\| PubMed ID: 24950238	2014
Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis. Frontiers in chemistry\| PubMed ID: 24959531	2014
Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis. New biotechnology\| PubMed ID: 25017988	2014
Characterizing and alleviating substrate limitations for improved in vitro ribosome construction. ACS synthetic biology\| PubMed ID: 25079899	2015
Cell-free metabolic engineering: biomanufacturing beyond the cell. Biotechnology journal\| PubMed ID: 25319678	2015
Controlled assembly of artificial protein-protein complexes via DNA duplex formation. Bioconjugate chemistry\| PubMed ID: 25603321	2015
High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Scientific reports\| PubMed ID: 25727242	2015
Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1. Chembiochem : a European journal of chemical biology\| PubMed ID: 25737329	2015
pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders. Protein engineering, design & selection : PEDS\| PubMed ID: 25877663	2015
Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction. Nucleic acids research\| PubMed ID: 25897121	2015
Energizing eukaryotic cell-free protein synthesis with glucose metabolism. FEBS letters\| PubMed ID: 26054976	2015
Self-Assembly of Temperature-Responsive Protein-Polymer Bioconjugates. Bioconjugate chemistry\| PubMed ID: 26083370	2015
Repurposing the translation apparatus for synthetic biology. Current opinion in chemical biology\| PubMed ID: 26186264	2015
Protein synthesis by ribosomes with tethered subunits. Nature\| PubMed ID: 26222032	2015
Robust production of recombinant phosphoproteins using cell-free protein synthesis. Nature communications\| PubMed ID: 26350765	2015
Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnology journal\| PubMed ID: 26356243	2016
A cell-free expression and purification process for rapid production of protein biologics. Biotechnology journal\| PubMed ID: 26427345	2016
Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol. Metabolic engineering\| PubMed ID: 26428449	2015
Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nature biotechnology\| PubMed ID: 26571098	2015
Evolution of translation initiation sequences using in vitro yeast ribosome display. Biotechnology and bioengineering\| PubMed ID: 26757179	2016
Editorial: Transforming biotechnology with synthetic biology. Biotechnology journal\| PubMed ID: 26844671	2016
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery. Metabolic engineering\| PubMed ID: 26996382	2016
Synthetic biology: Tailor-made genetic codes. Nature chemistry\| PubMed ID: 27001721	2016
Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes. Chemical communications (Cambridge, England)\| PubMed ID: 27019994	2016
Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis. ACS synthetic biology\| PubMed ID: 27476989	2016
In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis. ACS synthetic biology\| PubMed ID: 27478992	2017
Depletion of Shine-Dalgarno Sequences Within Bacterial Coding Regions Is Expression Dependent. G3 (Bethesda, Md.)\| PubMed ID: 27605518	2016
Cell-Free Synthetic Biology: Engineering Beyond the Cell. Cold Spring Harbor perspectives in biology\| PubMed ID: 27742731	2016
NullSeq: A Tool for Generating Random Coding Sequences with Desired Amino Acid and GC Contents. PLoS computational biology\| PubMed ID: 27835644	2016
Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins. Biotechnology and bioengineering\| PubMed ID: 27987323	2017
Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency. Open biology\| PubMed ID: 28100663	2017
Establishing a high yielding streptomyces-based cell-free protein synthesis system. Biotechnology and bioengineering\| PubMed ID: 28112394	2017
Cogenerating Synthetic Parts toward a Self-Replicating System. ACS synthetic biology\| PubMed ID: 28330337	2017
Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies. ACS synthetic biology\| PubMed ID: 28350472	2017
Repurposing ribosomes for synthetic biology. Current opinion in chemical biology\| PubMed ID: 28869851	2017
A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases. Methods in enzymology\| PubMed ID: 28935112	2017
Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis. Synthetic and systems biotechnology\| PubMed ID: 29062921	2016
Controlling cell-free metabolism through physiochemical perturbations. Metabolic engineering\| PubMed ID: 29155060	2018
Editorial overview: Synthetic biology: Frontiers in synthetic biology. Current opinion in chemical biology\| PubMed ID: 29156192	2017
A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases. Biotechnology and bioengineering\| PubMed ID: 29178580	2018
Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands. Molecular biology and evolution\| PubMed ID: 29220489	2017
Toward an orthogonal central dogma. Nature chemical biology\| PubMed ID: 29337969	2018
A novel framework for evaluating the performance of codon usage bias metrics. Journal of the Royal Society, Interface\| PubMed ID: 29386398	2018
Incorporation of Nonproteinogenic Amino Acids in Class I and II Lantibiotics. ACS chemical biology\| PubMed ID: 29439566	2018
How many human proteoforms are there? Nature chemical biology\| PubMed ID: 29443976	2018
A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology. Journal of the American Chemical Society\| PubMed ID: 29480720	2018
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids. Nature communications\| PubMed ID: 29572528	2018
Engineered Ribosomes for Basic Science and Synthetic Biology. Annual review of chemical and biomolecular engineering\| PubMed ID: 29589973	2018
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases. Nature chemical biology\| PubMed ID: 29736039	2018
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery. Nature communications\| PubMed ID: 30002445	2018
BioBits™ Explorer: A modular synthetic biology education kit. Science advances\| PubMed ID: 30083608	2018
BioBits™ Bright: A fluorescent synthetic biology education kit. Science advances\| PubMed ID: 30083609	2018
Within-Gene Shine-Dalgarno Sequences Are Not Selected for Function. Molecular biology and evolution\| PubMed ID: 30085185	2018
Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery. Nature communications\| PubMed ID: 30127449	2018
Cell-Free Synthetic Biology for Pathway Prototyping. Methods in enzymology\| PubMed ID: 30173768	2018
Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response. mSystems\| PubMed ID: 30320218	2018
Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry. ACS synthetic biology\| PubMed ID: 30596483	2019