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Escherichia coli-Based Cell-Free Protein Synthesis

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

Article DOI: 10.3791/58882-v
February 25th, 2019


Summary February 25th, 2019

This protocol details the steps, costs, and equipment necessary to generate E. coli-based cell extracts and implement in vitro protein synthesis reactions within 4 days or less. To leverage the flexible nature of this platform for broad applications, we discuss reaction conditions that can be adapted and optimized.


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