isolation-culture-characterization-differentiation-human-muscle

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

The use of primary human tissue and cells is ideal for the investigation of biological and physiological processes such as the skeletal muscle ...

Cornell University

Peptide YY (PYY) is considered a gut peptide with roles in post-prandial appetite and glucose regulation. Circulating PYY protein levels increase during aerobic exercise. Furthermore, people who have greater increases in muscle progenitor cells (MPCs), the adult stem cell population responsible for skeletal muscle (SkM) repair, after resistance training have higher PYY transcript levels in SkM prior to training. Currently, examination of PYY expression patterns in SkM and/or MPCs is lacking. Our objective was to identify the expression patterns of PYY in SkM and MPCs. PYY and the associated Y receptors were analyzed in SkM biopsy tissue and cultured MPCs from young and old human participants. Additional experiments to assess the role and regulation of PYY in MPCs were performed. In SkM, PYY and one of the three Y receptors (Y1r) were detectable, but expression patterns were not affected by age. In expanding MPCs, PYY and all three Y receptor (Y1r, Y2r, and Y5r) proteins were expressed in a temporal fashion with young MPCs having greater levels of Y receptors at various time points. Exogenous PYY did not affect MPC population expansion. MPC PYY levels increased following the metabolic stimulus, 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR), but were not affected by the inflammatory stimulus, tumor necrosis factor alpha (TNFα). In conclusion, PYY and Y receptor expression are not impacted by age in SkM tissue but are reduced in old  young expanding MPCs. Furthermore, endogenous PYY production is stimulated by low energy states and thus may be integral for skeletal muscle and MPC responses to metabolic stimuli.

Jamie Blum

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