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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

DOI:
10.3791/59580-v

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00:08 min

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August 23, 2019

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, , , , ,
1Division of Nutritional Sciences,Cornell University, 2Meinig School of Biomedical Engineering,Cornell University

Chapters

  • 00:04Title
  • 00:59Human Muscle Progenitor Cell (hMPC) Isolation
  • 03:11Imaging Cytometer hMPC Culture Characterization
  • 05:52Results: Representative hMPC Characterization
  • 07:21Conclusion

Summary

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We present techniques for isolating, culturing, characterizing, and differentiating human primary muscle progenitor cells (hMPCs) obtained from skeletal muscle biopsy tissue. hMPCs obtained and characterized through these methods can be used to subsequently address research questions related to human myogenesis and skeletal muscle regeneration.

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Keywords: Muscle Progenitor CellsSkeletal Muscle RegenerationBiopsyIsolationCultureCharacterizationDifferentiationPax7CD56CD29

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