Jason Moffat

Donnelly Centre, University of Toronto

Research Area

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 14	2019

Other Publications

Article	Year
Cyclin partners determine Pho85 protein kinase substrate specificity in vitro and in vivo: control of glycogen biosynthesis by Pcl8 and Pcl10. Molecular and cellular biology\| PubMed ID: 9584169	1998
Mammalian Cdk5 is a functional homologue of the budding yeast Pho85 cyclin-dependent protein kinase. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 10588725	1999
Transcriptional coregulation by the cell integrity mitogen-activated protein kinase Slt2 and the cell cycle regulator Swi4. Molecular and cellular biology\| PubMed ID: 11533240	2001
Dissection of a complex phenotype by functional genomics reveals roles for the yeast cyclin-dependent protein kinase Pho85 in stress adaptation and cell integrity. Molecular and cellular biology\| PubMed ID: 12077337	2002
Ac'septin' a signal: kinase regulation by septins. Developmental cell\| PubMed ID: 14536051	2003
Late-G1 cyclin-CDK activity is essential for control of cell morphogenesis in budding yeast. Nature cell biology\| PubMed ID: 14688790	2004
Exploration of essential gene functions via titratable promoter alleles. Cell\| PubMed ID: 15242642	2004
A role for the TGFbeta-Par6 polarity pathway in breast cancer progression. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 19667198	2009
Dual regulation by pairs of cyclin-dependent protein kinases and histone deacetylases controls G1 transcription in budding yeast. PLoS biology\| PubMed ID: 19823668	2009
The ubiquitin-activating enzyme E1 as a therapeutic target for the treatment of leukemia and multiple myeloma. Blood\| PubMed ID: 20075161	2010
A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency. Molecular & cellular proteomics : MCP\| PubMed ID: 20305087	2010
Selective targeting of neuroblastoma tumour-initiating cells by compounds identified in stem cell-based small molecule screens. EMBO molecular medicine\| PubMed ID: 20721990	2010
SATB2 augments ΔNp63α in head and neck squamous cell carcinoma. EMBO reports\| PubMed ID: 20829881	2010
A comprehensive platform for highly multiplexed mammalian functional genetic screens. BMC genomics\| PubMed ID: 21548937	2011
Pooled lentiviral shRNA screening for functional genomics in mammalian cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 21877282	2011
SnapShot: High-throughput sequencing applications. Cell\| PubMed ID: 21925324	2011
CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope. The Journal of biological chemistry\| PubMed ID: 21937449	2011
Inhibition of mitochondrial translation as a therapeutic strategy for human acute myeloid leukemia. Cancer cell\| PubMed ID: 22094260	2011
COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines. Nucleic acids research\| PubMed ID: 22102578	2012
Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B. Transcription\| PubMed ID: 22231121	2011
The mixed lineage leukemia (MLL) fusion-associated gene AF4 promotes CD133 transcription. Cancer research\| PubMed ID: 22337994	2012
Essential gene profiles in breast, pancreatic, and ovarian cancer cells. Cancer discovery\| PubMed ID: 22585861	2012
A versatile lentiviral expression system to identify mammalian protein-protein interactions. Methods (San Diego, Calif.)\| PubMed ID: 22713554	2012
Developmentally programmed nuclear destruction during yeast gametogenesis. Developmental cell\| PubMed ID: 22727375	2012
Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nature cell biology\| PubMed ID: 22842922	2012
Suppression of cancer progression by MGAT1 shRNA knockdown. PloS one\| PubMed ID: 22957033	2012
Regulation of CD133 by HDAC6 promotes β-catenin signaling to suppress cancer cell differentiation. Cell reports\| PubMed ID: 23084749	2012
A strategy for modulation of enzymes in the ubiquitin system. Science (New York, N.Y.)\| PubMed ID: 23287719	2013
MBNL proteins repress ES-cell-specific alternative splicing and reprogramming. Nature\| PubMed ID: 23739326	2013
RNA interference screens to uncover membrane protein biology. Briefings in functional genomics\| PubMed ID: 23793263	2013
Miniature short hairpin RNA screens to characterize antiproliferative drugs. G3 (Bethesda, Md.)\| PubMed ID: 23797109	2013
Integrated genomic, transcriptomic, and RNA-interference analysis of genes in somatic copy number gains in pancreatic ductal adenocarcinoma. Pancreas\| PubMed ID: 23851435	2013
Targeting HER2+ breast cancer cells: lysosomal accumulation of anti-HER2 antibodies is influenced by antibody binding site and conjugation to polymeric nanoparticles. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 23880472	2013
A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Molecular systems biology\| PubMed ID: 24104479	2013
shRNA kinome screen identifies TBK1 as a therapeutic target for HER2+ breast cancer. Cancer research\| PubMed ID: 24487029	2014
The RhoGEF GEF-H1 is required for oncogenic RAS signaling via KSR-1. Cancer cell\| PubMed ID: 24525234	2014
Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 24550280	2014
Post-translational regulation of CD133 by ATase1/ATase2-mediated lysine acetylation. Journal of molecular biology\| PubMed ID: 24556617	2014
CD133-targeted niche-dependent therapy in cancer: a multipronged approach. The American journal of pathology\| PubMed ID: 24589338	2014
The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nature methods\| PubMed ID: 24658140	2014
A genome scale overexpression screen to reveal drug activity in human cells. Genome medicine\| PubMed ID: 24944581	2014
Measuring error rates in genomic perturbation screens: gold standards for human functional genomics. Molecular systems biology\| PubMed ID: 24987113	2014
A systematic approach to identify novel cancer drug targets using machine learning, inhibitor design and high-throughput screening. Genome medicine\| PubMed ID: 25165489	2014
Differential regulation of FGFR3 by PTPN1 and PTPN2. Proteomics\| PubMed ID: 25311528	2015
Coiled-coil domain containing 68 (CCDC68) demonstrates a tumor-suppressive role in pancreatic ductal adenocarcinoma. Oncogene\| PubMed ID: 25381825	2015
RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR. Molecular & cellular proteomics : MCP\| PubMed ID: 25825526	2015
Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis. Cell\| PubMed ID: 26046442	2015
CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae. G3 (Bethesda, Md.)\| PubMed ID: 26048563	2015
Inhibition of the Mitochondrial Protease ClpP as a Therapeutic Strategy for Human Acute Myeloid Leukemia. Cancer cell\| PubMed ID: 26058080	2015
INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.)\| PubMed ID: 26068852	2015
Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes. Cell\| PubMed ID: 26186192	2015
Extensive mapping of an innate immune network with CRISPR. Molecular systems biology\| PubMed ID: 26208794	2015
A High Through-put Platform for Recombinant Antibodies to Folded Proteins. Molecular & cellular proteomics : MCP\| PubMed ID: 26290498	2015
Identification of P450 Oxidoreductase as a Major Determinant of Sensitivity to Hypoxia-Activated Prodrugs. Cancer research\| PubMed ID: 26297733	2015
Selection of recombinant anti-SH3 domain antibodies by high-throughput phage display. Protein science : a publication of the Protein Society\| PubMed ID: 26332758	2015
Genome-wide RNAi analysis reveals that simultaneous inhibition of specific mevalonate pathway genes potentiates tumor cell death. Oncotarget\| PubMed ID: 26353928	2015
Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint. Genetics\| PubMed ID: 26354767	2015
High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell\| PubMed ID: 26627737	2015
A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell\| PubMed ID: 26638075	2015
Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells. Cell reports\| PubMed ID: 26673326	2015
Identification of RNF168 as a PML nuclear body regulator. Journal of cell science\| PubMed ID: 26675234	2016
SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination. Nature\| PubMed ID: 26700805	2016
Functional Genomic Landscape of Human Breast Cancer Drivers, Vulnerabilities, and Resistance. Cell\| PubMed ID: 26771497	2016
Automated inference procedure for the determination of cell growth parameters. Physical review. E\| PubMed ID: 26871096	2016
Cytokinetic effects of Wee1 disruption in pancreatic cancer. Cell cycle (Georgetown, Tex.)\| PubMed ID: 26890070	2016
Pooled screening for antiproliferative inhibitors of protein-protein interactions. Nature chemical biology\| PubMed ID: 26900867	2016
System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes. Molecular cell\| PubMed ID: 26949039	2016
Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1-F-box interface. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 26976582	2016
BAGEL: a computational framework for identifying essential genes from pooled library screens. BMC bioinformatics\| PubMed ID: 27083490	2016
The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination through ATM and DNAPK-Mediated γH2AX Formation. Cell reports\| PubMed ID: 27160905	2016
Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized med... BMC cancer\| PubMed ID: 27422173	2016
Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching. Scientific reports\| PubMed ID: 27853268	2016
Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors. Nature medicine\| PubMed ID: 27869803	2017
Design and Experimental Validation of Small Activating RNAs Targeting an Exogenous Promoter in Human Cells. ACS synthetic biology\| PubMed ID: 28033709	2017
Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors. Molecular cell\| PubMed ID: 28157508	2017
SMYD2 lysine methyltransferase regulates leukemia cell growth and regeneration after genotoxic stress. Oncotarget\| PubMed ID: 28187429	2017
Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10. Journal of molecular biology\| PubMed ID: 28587923	2017
Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens. G3 (Bethesda, Md.)\| PubMed ID: 28655737	2017
RNAi screen identifies essential regulators of human brain metastasis-initiating cells. Acta neuropathologica\| PubMed ID: 28766011	2017
Emerging and evolving concepts in gene essentiality. Nature reviews. Genetics\| PubMed ID: 29033457	2018
Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 29476010	2018
The Inhibitory NKR-P1B:Clr-b Recognition Axis Facilitates Detection of Oncogenic Transformation and Cancer Immunosurveillance. Cancer research\| PubMed ID: 29691253	2018
Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48. Nature communications\| PubMed ID: 29891926	2018
Cotargeting Ephrin Receptor Tyrosine Kinases A2 and A3 in Cancer Stem Cells Reduces Growth of Recurrent Glioblastoma. Cancer research\| PubMed ID: 29945963	2018
CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature\| PubMed ID: 29973717	2018
The shieldin complex mediates 53BP1-dependent DNA repair. Nature\| PubMed ID: 30022168	2018
A synthetic anti-Frizzled antibody engineered for broadened specificity exhibits enhanced anti-tumor properties. mAbs\| PubMed ID: 30183492	2018
EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 30324522	2019
Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 30324523	2019
Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons. Molecular cell\| PubMed ID: 30388412	2018
Protocadherin-1 is essential for cell entry by New World hantaviruses. Nature\| PubMed ID: 30464266	2018
Functional genomic characterization of a synthetic anti-HER3 antibody reveals a role for ubiquitination by RNF41 in the anti-proliferative response. The Journal of biological chemistry\| PubMed ID: 30523157	2019
A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations. Scientific reports\| PubMed ID: 30696911	2019
Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15. Structure (London, England : 1993)\| PubMed ID: 30713027	2019
Yeast Two-Hybrid Analysis for Ubiquitin Variant Inhibitors of Human Deubiquitinases. Journal of molecular biology\| PubMed ID: 30763569	2019
Structure-guided design fine-tunes pharmacokinetics, tolerability, and antitumor profile of multispecific frizzled antibodies. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 30894493	2019
Global Genetic Networks and the Genotype-to-Phenotype Relationship. Cell\| PubMed ID: 30901552	2019
Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Cell reports\| PubMed ID: 30970261	2019
Genetic interaction networks in cancer cells. Current opinion in genetics & development\| PubMed ID: 30974317	2019
Bmi1 regulates human glioblastoma stem cells through activation of differential gene networks in CD133+ brain tumor initiating cells. Journal of neuro-oncology\| PubMed ID: 31115870	2019
Identifying chemogenetic interactions from CRISPR screens with drugZ. Genome medicine\| PubMed ID: 31439014	2019
Tailored tetravalent antibodies potently and specifically activate Wnt/Frizzled pathways in cells, organoids and mice. eLife\| PubMed ID: 31452509	2019
CRISPR screens are feasible in TP53 wild-type cells. Molecular systems biology\| PubMed ID: 31464370	2019