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JoVE Journal
Genetics

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
 

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Article DOI: 10.3791/59780-v
September 4th, 2019

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Summary September 4th, 2019

CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.

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