implantation-human-sized-coronary-stents-into-rat-abdominal-aorta

Implantation of Human-Sized Coronary Stents into Rat Abdominal Aorta Using a Trans-Femoral Access

Percutaneous coronary intervention (PCI), combined with the deployment of a coronary stent, represents the gold standard in interventional treatment of ...

University Hospital RWTH Aachen

Due to the drastic shortage of organ donors, clinicians are increasingly considering the use of deceased after cardiac death donors (DCD). Compatible solutes like Ectoine and Hydroxyectoine are produced by extremophilic bacteria as a cell protectant to survive in harsh environments. We hypothesized that the addition of Hydroxyectoine to Histidine-Tryptophan-Ketoglutarate solution (HTK) could ameliorate cold ischemic preservation injury of DCD livers.

Hydroxyectoine ameliorates preservation injury in deceased after cardiac death donors in experimental liver grafts.

Worldwide shortage of donor organs has increased the use of donation after cardiac death (DCD). The aim of this study was to analyze the best time point for venous systemic oxygen persufflation (VSOP) supplemented with nitric oxide (NO) gas during the 1st and 24th hour of cold storage (CS) in warm ischemia (WI)-damaged experimental liver grafts.

Optimal Timing for Venous Systemic Oxygen Persufflation Supplemented with Nitric Oxide Gas in Cold-Stored, Warm Ischemia-Damaged Experimental Liver Grafts.

To investigate the role of CGS21680, a selective adenosine A2A receptor agonist, on a bile-duct-ligated cirrhotic liver resection model in rats.

Protective Effects of Adenosine Receptor Agonist in a Cirrhotic Liver Resection Model.

In vivo endothelialization and neointimal hyperplasia assessment after angioplasty of sheep carotid artery with a novel polycarbonate polyurethane patch.

The long-term success of coronary stent implantation is limited by in-stent restenosis (ISR). In spite of a broad variety of animal models available, an ideal high-throughput model of ISR has been lacking. Apolipoprotein E (apoE) deficient rats enable the evaluation of human-sized coronary stents while at the same time providing an atherogenic phenotype. Whereas apoE deficient rats have been proposed as animal model of atherosclerosis, to date it is unknown whether they also develop pronounced ISR. We sought to assess ISR after abdominal aorta stent implantation in apoE deficient rats. A total of 42 rats (16 wildtype, 13 homozygous apoE and 13 heterozygous apoE rats) underwent abdominal aorta stent implantation. After 28 days blood samples were analyzed to characterize lipid profiles. ISR was assessed by histomorphometric means. Homozygous apoE rats exhibited significantly higher total cholesterol and low-density cholesterol levels than wildtype apoE and heterozygous apoE rats. ISR was significantly pronounced in homozygous apoE rats as compared to wildtype apoE (p = <0.0001) and heterozygous apoE rats (p = 0.0102) on western diet. Abdominal aorta stenting of apoE rats is a reliable model to investigate ISR after stent implantation and thus can be used for the evaluation of novel stent concepts. Apolipoprotein E (apoE) deficient rats have been proposed as animal model of atherosclerosis. We investigated the development of restenosis 28 days after stent implantation into the abdominal aorta of wildtype apoE, homozygous apoE and heterozygous apoE rats, respectively. Homozygous apoE rats exhibited significantly higher LDL and significantly lower HDL cholesterol levels compared to wildtype apoE and heterozygous apoE rats. Restenosis after stent implantation was significantly pronounced in western-diet-fed homozygous apoE rats, accompanied by a significantly increased neointimal thickness. Thus, apoE knockout rats exhibit elevated restenosis and might provide a novel tool for testing of innovative stent concepts.

Apolipoprotein E deficient rats generated via zinc-finger nucleases exhibit pronounced in-stent restenosis.

In patients with cardiovascular events, such as myocardial infarction or aortic dissection without known risk factors for cardiovascular disease, neoplastic disease should be considered as a differential diagnosis. In this report, we present a case of a 51-year old man with previously undiagnosed non-small lung cancer leading to fatal cardiovascular complications due to hemovascular spread, diagnosed post-mortem. This case illustrates the value of autopsy in unexpected deaths.

Article	Year
Hydroxyectoine ameliorates preservation injury in deceased after cardiac death donors in experimental liver grafts. Annals of transplantation\| PubMed ID: 24727658	2014
Optimal Timing for Venous Systemic Oxygen Persufflation Supplemented with Nitric Oxide Gas in Cold-Stored, Warm Ischemia-Damaged Experimental Liver Grafts. European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes\| PubMed ID: 27271697	2016
Protective Effects of Adenosine Receptor Agonist in a Cirrhotic Liver Resection Model. Hepatitis monthly\| PubMed ID: 27799962	2016
In vivo endothelialization and neointimal hyperplasia assessment after angioplasty of sheep carotid artery with a novel polycarbonate polyurethane patch. Journal of biomaterials applications\| PubMed ID: 31088184	2019
Apolipoprotein E deficient rats generated via zinc-finger nucleases exhibit pronounced in-stent restenosis. Scientific reports\| PubMed ID: 31796798	2019
An unusual case of aortic metastasis from lung cancer. Discoveries (Craiova, Romania)\| PubMed ID: 32309623	2020

Mamdouh Afify

Biography

JoVE Journal Publications

Other Publications