Jacek Otlewski

Department of Protein Engineering, University of Wroclaw

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 2	2021

Other Publications

Article	Year
Predicting the reactivity of proteins from their sequence alone: Kazal family of protein inhibitors of serine proteinases. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 11171964	2001
Amino-acid substitutions at the fully exposed P1 site of bovine pancreatic trypsin inhibitor affect its stability. Protein science : a publication of the Protein Society\| PubMed ID: 11274462	2001
Analysis of serine proteinase-inhibitor interaction by alanine shaving. Protein science : a publication of the Protein Society\| PubMed ID: 11910024	2002
Thermodynamics of single peptide bond cleavage in bovine pancreatic trypsin inhibitor (BPTI). Protein science : a publication of the Protein Society\| PubMed ID: 11910035	2002
NMR structures of two variants of bovine pancreatic trypsin inhibitor (BPTI) reveal unexpected influence of mutations on protein structure and stability. Journal of molecular biology\| PubMed ID: 12206780	2002
Hydrogen bonds in human ubiquitin reflected in temperature coefficients of amide protons. Journal of magnetic resonance (San Diego, Calif. : 1997)\| PubMed ID: 12323135	2002
Importance of alpha-helix N-capping motif in stabilization of betabetaalpha fold. Protein science : a publication of the Protein Society\| PubMed ID: 12761399	2003
Amyloid-forming peptides selected proteolytically from phage display library. Protein science : a publication of the Protein Society\| PubMed ID: 12876317	2003
PDZ domains - common players in the cell signaling. Acta biochimica Polonica\| PubMed ID: 14739991	2003
Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements. Protein science : a publication of the Protein Society\| PubMed ID: 15044735	2004
Design of fully active FGF-1 variants with increased stability. Protein engineering, design & selection : PEDS\| PubMed ID: 15469994	2004
Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants. Journal of molecular biology\| PubMed ID: 15544809	2004
The many faces of protease-protein inhibitor interaction. The EMBO journal\| PubMed ID: 15775973	2005
Highly stable mutants of human fibroblast growth factor-1 exhibit prolonged biological action. Journal of molecular biology\| PubMed ID: 16126225	2005
The dimerization mechanism of LIS1 and its implication for proteins containing the LisH motif. Journal of molecular biology\| PubMed ID: 16445939	2006
The molecular basis of RhoA specificity in the guanine nucleotide exchange factor PDZ-RhoGEF. The Journal of biological chemistry\| PubMed ID: 16954208	2006
Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin. The Plant cell\| PubMed ID: 16998071	2006
Novel peptide recognized by RhoA GTPase. Acta biochimica Polonica\| PubMed ID: 17019437	2006
Structural requirements of FGF-1 for receptor binding and translocation into cells. Biochemistry\| PubMed ID: 17176056	2006
Bivalent peptides as models for multimeric targets of PDZ domains. Chembiochem : a European journal of chemical biology\| PubMed ID: 17279591	2007
Expression, purification and crystallization of cysteine-rich human protein muskelin in Escherichia coli. Protein expression and purification\| PubMed ID: 18455433	2008
Degenerate specificity of PDZ domains from RhoA-specific nucleotide exchange factors PDZRhoGEF and LARG. Acta biochimica Polonica\| PubMed ID: 18542831	2008
Dissecting the thermodynamics of GAP-RhoA interactions. Journal of structural biology\| PubMed ID: 18929667	2009
Structure of a highly stable mutant of human fibroblast growth factor 1. Acta crystallographica. Section D, Biological crystallography\| PubMed ID: 19153468	2009
Increased protein stability of FGF1 can compensate for its reduced affinity for heparin. The Journal of biological chemistry\| PubMed ID: 19574212	2009
The high Zn(II) affinity of the tetracysteine tag affects its fluorescent labeling with biarsenicals. Chembiochem : a European journal of chemical biology\| PubMed ID: 20440728	2010
Longer action means better drug: tuning up protein therapeutics. Biotechnology advances\| PubMed ID: 21443940	2011
Design and characteristics of a stable protein scaffold for specific binding based on variable lymphocyte receptor sequences. Biochimica et biophysica acta\| PubMed ID: 21621013	2011
PDZ domain from Dishevelled -- a specificity study. Acta biochimica Polonica\| PubMed ID: 21666888	2011
Tailoring small proteins towards biomedical applications. Current pharmaceutical biotechnology\| PubMed ID: 21902634	2011
Selection and characterization of human antibody fragments specific for psoriasin - a cancer associated protein. Biochemical and biophysical research communications\| PubMed ID: 22342672	2012
Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability. Journal of inorganic biochemistry\| PubMed ID: 22922308	2012
A novel, stable, helical scaffold as an alternative binder - construction of phage display libraries. Acta biochimica Polonica\| PubMed ID: 23032749	2012
FGF1-gold nanoparticle conjugates targeting FGFR efficiently decrease cell viability upon NIR irradiation. International journal of nanomedicine\| PubMed ID: 23226697	2012
Aptamers: molecules of great potential. Biotechnology advances\| PubMed ID: 23632375	2013
Protease resistant variants of FGF1 with prolonged biological activity. Protein and peptide letters\| PubMed ID: 24304385	2014
Design, expression and characterization of a highly stable tetratricopeptide-based protein scaffold for phage display application. Acta biochimica Polonica\| PubMed ID: 24350305	2013
Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles. International journal of nanomedicine\| PubMed ID: 24379664	2014
Nucleolin regulates phosphorylation and nuclear export of fibroblast growth factor 1 (FGF1). PloS one\| PubMed ID: 24595027	2014
Efficient production and purification of extracellular domain of human FGFR-Fc fusion proteins from Chinese hamster ovary cells. Protein expression and purification\| PubMed ID: 24727156	2014
Current methods for the synthesis of homogeneous antibody-drug conjugates. Biotechnology advances\| PubMed ID: 25981886	2015
Selection and Characterization of Single Chain Antibody Fragments Specific for Hsp90 as a Potential Cancer Targeting Molecule. International journal of molecular sciences\| PubMed ID: 26307975	2015
Selection of specific interactors from phage display library based on sea lamprey variable lymphocyte receptor sequences. Biochimica et biophysica acta\| PubMed ID: 26391289	2015
Identification of new FGF1 binding partners-Implications for its intracellular function. IUBMB life\| PubMed ID: 26840910	2016
A Novel Affibody-Auristatin E Conjugate With a Potent and Selective Activity Against HER2+ Cell Lines. Journal of immunotherapy (Hagerstown, Md. : 1997)\| PubMed ID: 27227324	2016
Design and characteristics of cytotoxic fibroblast growth factor 1 conjugate for fibroblast growth factor receptor-targeted cancer therapy. Drug design, development and therapy\| PubMed ID: 27563235	2016
A Conjugate Based on Anti-HER2 Diaffibody and Auristatin E Targets HER2-Positive Cancer Cells. International journal of molecular sciences\| PubMed ID: 28216573	2017
Anti-FGFR1 aptamer-tagged superparamagnetic conjugates for anticancer hyperthermia therapy. International journal of nanomedicine\| PubMed ID: 28442904	2017
High-Affinity Internalizing Human scFv-Fc Antibody for Targeting FGFR1-Overexpressing Lung Cancer. Molecular cancer research : MCR\| PubMed ID: 28483948	2017
Site-specific conjugation of fibroblast growth factor 2 (FGF2) based on incorporation of alkyne-reactive unnatural amino acid. Bioorganic & medicinal chemistry\| PubMed ID: 28522266	2017
High-Yield Site-Specific Conjugation of Fibroblast Growth Factor 1 with Monomethylauristatin E via Cysteine Flanked by Basic Residues. Bioconjugate chemistry\| PubMed ID: 28598150	2017
The autoinhibitory function of D1 domain of FGFR1 goes beyond the inhibition of ligand binding. The international journal of biochemistry & cell biology\| PubMed ID: 28652212	2017
Antibody-induced dimerization of FGFR1 promotes receptor endocytosis independently of its kinase activity. Scientific reports\| PubMed ID: 28769084	2017
Design and In Vitro Evaluation of a Cytotoxic Conjugate Based on the Anti-HER2 Affibody Fused to the Fc Fragment of IgG1. International journal of molecular sciences\| PubMed ID: 28771178	2017
Generation of high-affinity, internalizing anti-FGFR2 single-chain variable antibody fragment fused with Fc for targeting gastrointestinal cancers. PloS one\| PubMed ID: 29420662	2018
High Affinity Promotes Internalization of Engineered Antibodies Targeting FGFR1. International journal of molecular sciences\| PubMed ID: 29748524	2018
Cytotoxic Conjugates of Fibroblast Growth Factor 2 (FGF2) with Monomethyl Auristatin E for Effective Killing of Cells Expressing FGF Receptors. ACS omega\| PubMed ID: 30023704	2017
FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect towards Cancer Cells Overproducing FGF Receptor 1. International journal of molecular sciences\| PubMed ID: 30029518	2018
Translocation of Exogenous FGF1 and FGF2 Protects the Cell against Apoptosis Independently of Receptor Activation. Journal of molecular biology\| PubMed ID: 30099027	2018
Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity. International journal of molecular sciences\| PubMed ID: 30134556	2018
Correction: Serwotka-Suszczak, A. M. et al. A Conjugate Based on Anti-HER2 Diaffibody and Auristatin E Targets HER2-Positive Cancer Cells. Int. J. Mol. Sci. 2017, 18, 401. International journal of molecular sciences\| PubMed ID: 30463379	2018
Targeting Cellular Trafficking of Fibroblast Growth Factor Receptors as a Strategy for Selective Cancer Treatment. Journal of clinical medicine\| PubMed ID: 30577533	2018
Identification of a peptide antagonist of the FGF1-FGFR1 signaling axis by phage display selection. FEBS open bio\| PubMed ID: 30968602	2019
Crosstalk between p38 and Erk 1/2 in Downregulation of FGF1-Induced Signaling. International journal of molecular sciences\| PubMed ID: 31013829	2019
Cross-Talk between Fibroblast Growth Factor Receptors and Other Cell Surface Proteins. Cells\| PubMed ID: 31091809	2019
Differential regulation of fibroblast growth factor receptor 1 trafficking and function by extracellular galectins. Cell communication and signaling : CCS\| PubMed ID: 31208421	2019
Low Stability of Integrin-Binding Deficient Mutant of FGF1 Restricts Its Biological Activity. Cells\| PubMed ID: 31443196	2019
FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner. Cell communication and signaling : CCS\| PubMed ID: 32357892	2020
Intracellular partners of fibroblast growth factors 1 and 2 - implications for functions. Cytokine & growth factor reviews\| PubMed ID: 32475760	2020
Site-Specific, Stoichiometric-Controlled, PEGylated Conjugates of Fibroblast Growth Factor 2 (FGF2) with Hydrophilic Auristatin Y for Highly Selective Killing of Cancer Cells Overproducing F... Molecular pharmaceutics\| PubMed ID: 32501706	2020
FGFR1 clustering with engineered tetravalent antibody improves the efficiency and modifies the mechanism of receptor internalization. Molecular oncology\| PubMed ID: 32511887	2020
Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential. International journal of molecular sciences\| PubMed ID: 32526859	2020
FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1. Cancers\| PubMed ID: 33076489	2020