blood-circuit-reconstruction-an-abdominal-mouse-heart-transplantation

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

The surgical technique of heterotopic abdominal heart transplantation in mice is a standard model for research in transplantation immunology. Here, the ...

a-modified-surgical-technique-for-kidney-transplantation-in-mice

A Modified Surgical Technique for Kidney Transplantation in Mice

Kidney transplantation in mice is a complicated and challenging surgery procedure. There are very few publications demonstrating the key steps of this ...

University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg

Friedrich-Alexander University Erlangen-Nürnberg

Allograft-specific regulatory T cells (T cells) are crucial for long-term graft acceptance after transplantation. Although adoptive T cell transfer has been proposed, major challenges include graft-specificity and stability. Thus, there is an unmet need for the direct induction of graft-specific T cells. We hypothesized a synergism of the immunotolerogenic effects of rapamycin (mTOR inhibition) and plerixafor (CXCR4 antagonist) for T cell induction. Thus, we performed fully-mismatched heart transplantations and found combination treatment to result in prolonged allograft survival. Moreover, fibrosis and myocyte lesions were reduced. Although less CD3 T cell infiltrated, higher T cell numbers were observed. Noteworthy, this was accompanied by a plerixafor-dependent plasmacytoid dendritic cells-(pDCs)-mobilization. Furthermore, in vivo pDC-depletion abrogated the plerixafor-mediated T cell number increase and reduced allograft survival. Our pharmacological approach allowed to increase T cell numbers due to pDC-mediated immune regulation. Therefore pDCs can be an attractive immunotherapeutic target in addition to plerixafor treatment.

ArticleTotal : 2	Year
Publication title Cited by 2	2021
Publication title Cited by 3	2022

Ida Allabauer

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