exploring-m6a-m5c-epitranscriptomes-upon-viral-infection-an-example

Exploring m<sup>6</sup>A and m<sup>5</sup>C Epitranscriptomes upon Viral Infection: an Example with HIV

The role of RNA modifications in biological processes has been the focus of an increasing number of studies in the last few years and is known nowadays as ...

Lausanne University Hospital and University of Lausanne

Single-cell analyses allow uncovering cellular heterogeneity, not only per se, but also in response to viral infection. Similarly, single cell transcriptome analyses (scRNA-Seq) can highlight specific signatures, identifying cell subsets with particular phenotypes, which are relevant in the understanding of virus-host interactions.

The use of single-cell RNA-Seq to understand virus-host interactions.

Despite effective treatment, HIV can persist in latent reservoirs, which represent a major obstacle toward HIV eradication. Targeting and reactivating latent cells is challenging due to the heterogeneous nature of HIV-infected cells. Here, we used a primary model of HIV latency and single-cell RNA sequencing to characterize transcriptional heterogeneity during HIV latency and reactivation. Our analysis identified transcriptional programs leading to successful reactivation of HIV expression.

Article	Year
The use of single-cell RNA-Seq to understand virus-host interactions. Current opinion in virology\| PubMed ID: 29558678	2018
Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity in Latent and Reactivated HIV-Infected Cells. Cell reports\| PubMed ID: 29694901	2018

Sara Cristinelli

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