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Exploring m6A and m5C Epitranscriptomes upon Viral Infection
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Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Article DOI: 10.3791/62426-v • 14:40 min • March 5th, 2022
March 5th, 2022 •
1, 2, 1
1Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 2Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics

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The role of RNA modifications in viral infections is just starting to be explored and could highlight new viral-host interaction mechanisms. In this work, we provide a pipeline to investigate m6A and m5C RNA modifications in the context of viral infections.

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M6A M5C Epitranscriptomes Viral Infection HIV Cells Noninfected Infected Condition Dataset Universal Infection HIV-based Vector GFP FACS Total RNA Extraction Trizol Chloroform Phase Separation Upper Phase Aqueous Phase Isopropanol RNA Precipitation RNA Pellet
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