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Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
JoVE Journal
Biology
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JoVE Journal Biology
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

DOI:
10.3791/62426-v

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14:40 min

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March 05, 2022

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, ,
1Institute of Microbiology,Lausanne University Hospital and University of Lausanne, 2Bioinformatics Core Facility,SIB Swiss Institute of Bioinformatics

Chapters

  • 00:00Introduction
  • 00:07Experimental Workflow
  • 00:38Total RNA Extraction
  • 02:22mRNA Isolation
  • 04:38mRNA Fragmentation
  • 06:09meRIP-Seq
  • 10:21Bisulfite Conversion
  • 12:47Results
  • 13:45Conclusion

Summary

Automatic Translation

Automatic Translation

The role of RNA modifications in viral infections is just starting to be explored and could highlight new viral-host interaction mechanisms. In this work, we provide a pipeline to investigate m6A and m5C RNA modifications in the context of viral infections.

Tags

Keywords: M6AM5CEpitranscriptomeViral InfectionHIVRNA ExtractionMRNA IsolationPoly-A SelectionRNA-seq

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