mapping-molecular-diffusion-plasma-membrane-multiple-target-tracing

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Our goal is to obtain a comprehensive description of molecular processes occurring at cellular membranes in different biological functions. We aim at ...

Parc scientifique de Luminy

Aix-Marseille University

We present the assets and constraints of using optical parametric oscillators (OPOs) to perform point scanning nonlinear microscopy and spectroscopy with special emphasis on coherent Raman spectroscopy. The different possible configurations starting with one OPO and two OPOs are described in detail and with comments that are intended to be practically useful for the user. Explicit examples on test samples such as nonlinear organic crystal, polystyrene beads, and fresh mouse tissues are given. Special emphasis is given to background-free coherent Raman anti-Stokes scattering (CARS) imaging, including CARS hyperspectral imaging in a fully automated mode with commercial OPOs.

Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview.

Performing label free coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) in endoscope imaging is a challenge, with huge potential clinical benefit. To date, this goal has remained inaccessible because of the inherent coherent Raman noise that is generated in the fiber itself. By developing double-clad hollow core photonic crystal fiber, we demonstrate coherent anti-Stokes Raman scattering and stimulated Raman scattering in an 'endoscope-like' scheme. Both the excitation beams and the collected CARS and SRS signals travel through the same fiber. No CARS and SRS signals are generated within the hollow core fiber even for temporally overlapping pump and Stokes beams, leading to excellent image quality. The CARS and SRS signals generated in the sample are coupled back into a high numerical aperture multimode cladding surrounding the central photonic crystal cladding. We demonstrate this scheme by imaging molecular vibrational bonds of organic crystal deposited on a glass surface.

Article	Year
Optics letters\| PubMed ID: 18197227	2008
Optics express\| PubMed ID: 19259187	2009
Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview. Journal of biomedical optics\| PubMed ID: 21361669	2011
Double-clad hollow core photonic crystal fiber for coherent Raman endoscope. Optics express\| PubMed ID: 21716497	2011

Cyrille Billaudeau

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