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1Institut National de la Santé et de la Recherche Médicale, UMR 631,Parc scientifique de Luminy, 2Centre National de la Recherche Scientifique, UMR 6102,Parc scientifique de Luminy, 3Centre d’Immunologie de Marseille-Luminy,Aix-Marseille University, 4École Centrale Marseille,Technopôle de Château-Gombert, 5Institut Fresnel,Aix-Marseille University, 6Centre National de la Recherche Scientifique, UMR 6133,Aix-Marseille University
Chapters
- 00:02Title
- 01:36Sample Preparation
- 04:14Setup
- 04:52Acquisition
- 05:43Analysis
- 10:08Conclusion
Multiple-Target Tracing is a homemade algorithm developed for tracking individually labeled molecules within the plasma membrane of living cells. Efficiently detecting, estimating and tracing molecules over time at high-density provide a user-friendly, comprehensive tool to investigate nanoscale membrane dynamics.
Tags
Molecular DiffusionPlasma MembraneMultiple-target Tracing (MTT)Cellular MembranesSingle-molecule LevelSingle-particle Tracking (SPT)VideomicroscopyMillisecond ResolutionNanometric ResolutionMembrane OrganizationCell Receptors LocalizationMobilityConfinementInteractionsExperimental OptimizationCell LabelingLabeling DensityMolecular DynamicsPeaks DetectionEstimation And ReconnectionImage AnalysisDeflation After DetectionMonte-Carlo SimulationsLabeling DensityNoise Value
