Alan A. Doucette

Department of Chemistry, Dalhousie University

Research Area

Biography

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JoVE Journal Publications

ArticleTotal : 2	Year
Publication title Cited by 8	2022
Publication title	2023

Other Publications

Article	Year
Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry. Journal of the American Society for Mass Spectrometry\| PubMed ID: 16517177	2006
Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation. Analytical chemistry\| PubMed ID: 18229945	2008
Rapid and effective focusing in a carrier ampholyte solution isoelectric focusing system: a proteome prefractionation tool. Journal of proteome research\| PubMed ID: 18284188	2008
Evaluation of a solution isoelectric focusing protocol as an alternative to ion exchange chromatography for charge-based proteome prefractionation. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences\| PubMed ID: 19246255	2009
Multiplexed size separation of intact proteins in solution phase for mass spectrometry. Analytical chemistry\| PubMed ID: 19572727	2009
A qualitative proteome investigation of the sediment portion of human urine: Implications in the biomarker discovery process. Proteomics. Clinical applications\| PubMed ID: 21136939	2009
Implications of partial tryptic digestion in organic-aqueous solvent systems for bottom-up proteome analysis. Analytica chimica acta\| PubMed ID: 21889634	2011
Intact proteome fractionation strategies compatible with mass spectrometry. Expert review of proteomics\| PubMed ID: 22087661	2011
Perfluorooctanoic acid and ammonium perfluorooctanoate: volatile surfactants for proteome analysis? Rapid communications in mass spectrometry : RCM\| PubMed ID: 22302492	2012
Chromatographic behaviour of peptides following dimethylation with H2/D2-formaldehyde: implications for comparative proteomics. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences\| PubMed ID: 23122402	2012
Critical assessment of the spectroscopic activity assay for monitoring trypsin activity in organic-aqueous solvent. Analytical biochemistry\| PubMed ID: 23333224	2013
A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC). Journal of chromatography. B, Analytical technologies in the biomedical and life sciences\| PubMed ID: 23435344	2013
GELFrEE fractionation combined with mass spectrometry for proteome analysis of secreted toxins from Enteropathogenic Escherichia coli (EPEC). Molecular and cellular probes\| PubMed ID: 23831145	2013
Maximizing recovery of water-soluble proteins through acetone precipitation. Analytica chimica acta\| PubMed ID: 24016582	2013
Dual LC-MS platform for high-throughput proteome analysis. Journal of proteome research\| PubMed ID: 24090060	2013
Proteomic analysis of rat proximal tubule cells following stretch-induced apoptosis in an in vitro model of kidney obstruction. Journal of proteomics\| PubMed ID: 24316357	2014
Reprint of "GELFrEE fractionation combined with mass spectrometry for proteome analysis of secreted toxins from Enteropathogenic Escherichia coli (EPEC)". Molecular and cellular probes\| PubMed ID: 24486296	2014
A two-stage spin cartridge for integrated protein precipitation, digestion and SDS removal in a comparative bottom-up proteomics workflow. Journal of proteomics\| PubMed ID: 25316050	2015
Resolubilization of precipitated intact membrane proteins with cold formic acid for analysis by mass spectrometry. Journal of proteome research\| PubMed ID: 25384094	2014
Comparison of sodium dodecyl sulfate depletion techniques for proteome analysis by mass spectrometry. Journal of chromatography. A\| PubMed ID: 26422304	2015
Preventing N- and O-formylation of proteins when incubated in concentrated formic acid. Proteomics\| PubMed ID: 26840995	2016
Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins though Transmembrane Electrophoresis. Journal of proteome research\| PubMed ID: 27376408	2016
Mass Spectrometry of Intact Proteins Reveals +98 u Chemical Artifacts Following Precipitation in Acetone. Journal of proteome research\| PubMed ID: 28088865	2017
Proteomic Workflows for Biomarker Identification Using Mass Spectrometry - Technical and Statistical Considerations during Initial Discovery. Proteomes\| PubMed ID: 28250400	2013
The benefits (and misfortunes) of SDS in top-down proteomics. Journal of proteomics\| PubMed ID: 28286130	2018
Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment. Proteomes\| PubMed ID: 28991197	2017
Membrane-Based SDS Depletion Ahead of Peptide and Protein Analysis by Mass Spectrometry. Proteomics\| PubMed ID: 29575800	2018
A robust strategy for proteomic identification of biomarkers of invasive phenotype complexed with extracellular heat shock proteins. Cell stress & chaperones\| PubMed ID: 31650515	2019
Developing front-end devices for improved sample preparation in MS-based proteome analysis. Journal of mass spectrometry : JMS\| PubMed ID: 31957906	2020
Rapid and Quantitative Protein Precipitation for Proteome Analysis by Mass Spectrometry. Journal of proteome research\| PubMed ID: 32195589	2020
Mass spectrometry profiling of low molecular weight proteins and peptides isolated by acetone precipitation. Analytica chimica acta\| PubMed ID: 33161983	2020
Correction to: A robust strategy for proteomic identification of biomarkers of invasive phenotype complexed with extracellular heat shock proteins. Cell stress & chaperones\| PubMed ID: 33355877	2021
Recent advances in top-down proteome sample processing ahead of MS analysis. Mass spectrometry reviews\| PubMed ID: 34047392	2021
Automated Electrokinetic Platform for High-Throughput Sodium Dodecyl Sulfate Depletion Ahead of Proteome Analysis by Mass Spectrometry. Analytical chemistry\| PubMed ID: 34644045	2021
Accelerated SDS depletion from proteins by transmembrane electrophoresis: Impacts of Joule heating. Electrophoresis\| PubMed ID: 29417596	2018
Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis. Biology\| PubMed ID: 36290348	2022