Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

These protocols are designed to maximize the recovery as well as the purity of proteins through solvent-based precipitation. We're gonna show you a couple tricks to perform precipitation in vials, as well as a semi-automated approach in a spin cartridge format. We have also optimized the precipitation protocol to be consistent and quick.

The whole protocol only takes a few minutes. These protocols demonstrate that solvent precipitation of proteins is ideal for sample prep ahead of mass spectrometry. The protocols will fit seamlessly into bottom up as well as top down pro workflows.