technical-refinement-bilateral-renal-ischemia-reperfusion-mouse-model

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Cardiac arrest poses a large public health burden. Acute kidney injury (AKI) is an adverse marker in survivors of cardiac arrest following the return of ...

MacKay Junior College of Medicine, Nursing, and Management

Mackay Memorial Hospital

Coronary heart disease is a leading cause of death in the world and therapy to reduce injury is still needed. The uncoupling of glycolysis and glucose oxidation induces lactate accumulation during myocardial ischemia/reperfusion (I/R) injury. Cell death occurs and finally leads to myocardial infarction. Caffeic acid, one of the major phenolic constituents in nature, acts as an antioxidant. Pyrrolidinyl caffeamide (PLCA), a new derivative of caffeic acid, was synthesized by our team. We aimed to investigate the effect of PLCA on hypoxia/reoxygenation (H/R) in neonatal rat ventricular myocytes (NRVM) and on myocardial I/R in rats.

Pyrrolidinyl caffeamide against ischemia/reperfusion injury in cardiomyocytes through AMPK/AKT pathways.

We have developed a graphitic carbon nitride covered vanadium oxide nanocomposite (VO@g-CN) by a simple sonochemical approach (50 kHz and 150 W/cm). Furthermore, the morphology and chemical composition of the VO@g-CN nanocomposite was carried out by X-rays diffractometry (XRD), transmission electron microscopy (TEM) and electrochemical impedance spectroscopy (EIS). Furthermore, the VO@g-CN nanocomposite modified electrode was investigate electrochemical behavior of the anticancer drug. Compared with bare SPCE, VO/SPCE and g-CN/SPCE, VO@g-CN modified SPCE showed highest current response towards anti-cancer drug (methotrexate). Furthermore, the modified sensor exhibits with a sharp peaks and wide linear range (0.025-273.15 μM) by using DPV with the sensitivity of 7.122 μA μM cm. Notably, we have achieved a nanomolar detection limit (13.26 nM) for the DPV detection of methotrexate. Further, the practicability of the VO@g-CN nanocomposite modified sensor can be used for real time sensing of methotrexate in drug and blood serum samples with good recover ranges. It has potential applications in routine analysis with high specificity, excellent reproducibility and good stability.

A sensitive electrochemical determination of chemotherapy agent using graphitic carbon nitride covered vanadium oxide nanocomposite; sonochemical approach.

Copper (Cu) based metal oxides have high electrocatalytic ability. In this work, we are synthesized stone-like cuprous oxide particles (CuO SNPs) covered on acid functionalized graphene oxide (GOS) sheets using ultrasonic process (50 kHz and 100 W). Besides, the chemical structural and crystalline analyses of CuO SNPs@GOS composites were characterized by transmission electron microscopy, X-ray crystallography and energy-dispersive X-ray spectroscopy. The CuO SNPs@GOS nanomaterials were tested towards detection of 8-hydroxydeoxyguanosine (8-OHdG) in biological samples. As expected CuO SNPs@GOS catalyst modified electrodes performed an outstanding catalytic ability on 8-hydroxydeoxyguanosine oxidation. 8-OHdG is oxidative stress biomarker. Further, it is noted that the detection performance of CuO SNPs@GOS coated electrodes and it's highly enhanced due to the synergistic effect of CuO SNPs and GOS. Besides, the modified materials provide more electro-active faces and as well as rapid electron transport pathway and shorten diffusion. Moreover, oxidation of 8-OHdG sensor is exploring a long linear or working range of 0.02-1465 µM and high sensitivity (8.75 nM). The viability of the CuO SNPs@GOS proposed electrochemical methods have tested, to find out 8-OHdG concentrations in biological fluids (blood serum and urine) with a satisfying recovery ranges.

Sonochemical preparation of carbon nanosheets supporting cuprous oxide architecture for high-performance and non-enzymatic electrochemical sensor in biological samples.

Nanoball-structured ferromagnetic zinc ferrite nanocrystals (ZnFeO NPs) entrapped with graphitic-carbon nitride (g-CN) was produced via straightforward and facile sonochemical synthetical technique (titanium probe; 100 W/cm and 50 KHz). The morphological (SEM), elemental (EDS), diffraction (XRD), XPS, and electrochemical studies (CV) have been carry out to verify the nanostructure and shape of the materials. The ZnFeO NPs/g-CN electrode (GCE) was constructed which displayed outstanding electrochemical ability towards toxic 4-nitrophenol (NTP). A sensitive, selective, reproducible, and durable electrochemical NTP sensor was developed by ZnFeO NPs/g-CN modified electrode. The modified sensor exhibited a high sensitivity and 4.17 nanomolars of LOD. It's greater than the LOD of previously reported NTP modified sensors. The real-time experiments of the modified electrochemical (ZnFeO NPs/g-CN electrode) sensor were successfully explained in various water (river and drinking) samples and its showed high standard recoveries. Therefore, sonochemical synthetical method and fabrication of modified electrode were developed this work based on environmental analysis of NTP sensor.

Ultrasonic-assisted preparation and characterization of magnetic ZnFeO/g-CN nanomaterial and their applications towards electrocatalytic reduction of 4-nitrophenol.

Article	Year
Pyrrolidinyl caffeamide against ischemia/reperfusion injury in cardiomyocytes through AMPK/AKT pathways. Journal of biomedical science\| PubMed ID: 25879197	2015
A sensitive electrochemical determination of chemotherapy agent using graphitic carbon nitride covered vanadium oxide nanocomposite; sonochemical approach. Ultrasonics sonochemistry\| PubMed ID: 31450375	2019
Sonochemical preparation of carbon nanosheets supporting cuprous oxide architecture for high-performance and non-enzymatic electrochemical sensor in biological samples. Ultrasonics sonochemistry\| PubMed ID: 32229388	2020
Ultrasonic-assisted preparation and characterization of magnetic ZnFeO/g-CN nanomaterial and their applications towards electrocatalytic reduction of 4-nitrophenol. Ultrasonics sonochemistry\| PubMed ID: 32505099	2020

Shih Yi Lee

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