Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Acute kidney injury is a major risk factor associated with high mortality in patients with cardiac arrest after return of circulation. This procedure can be performed to induce acute kidney injury. This protocol is to induce renal ischemia reperfusion accurately, securely, and consistently.

Demonstrating the procedure will be Huang Cheng-Wei, an assistant from my laboratory. To begin, place an anesthetized eight-week-old C57BL/6 male mouse in the prone position on a surgery platform with an electric blanket to maintain body temperature. Next, touch the back, find the lumbar spine, and look for costovertebral angles below both sides of the last rib.