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Chengdu University of Traditional Chinese Medicine

 Danggui Buxue decoction (DBD), consisting of Angelicae Sinensis Radix (ASR) and Astragali Radix (AR), is a famous prescription with the function of antivasoconstriction. This study intends to probe its mechanisms on the relaxation of the middle cerebral artery (MCA).  Vascular tension of rat MCA was measured using a DMT620 M system. First, the identical series of concentrations of DBD, ASR, and AR were added into resting KCl and U46619 preconstricted MCA. According to the compatibility ratio, their dilatation effects were further investigated on KCl and U46619 preconstricted vessels. Third, four K channel blockers were employed to probe the vasodilator mechanism on KCl-contracted MCA. We finally examined the effects of DBD, ASR, and AR on the vascular tone of U46619-contracted MCA in the presence or absence of Ca.  Data suggested that DBD, ASR, and AR can relax on KCl and U46619 precontracted MCA with no effects on resting vessels. The vasodilator effect of ASR was greater than those of DBD and AR on KCl-contracted MCA. For U46619-contracted MCA, ASR showed a stronger vasodilator effect than DBD and AR at low concentrations, but DBD was stronger than ASR at high concentrations. Amazingly, the vasodilator effect of DBD was stronger than that of AR at all concentrations on two vasoconstrictors which evoked MCA. The vasodilator effect of ASR was superior to that of DBD at a compatibility ratio on KCl-contracted MCA at low concentrations, while being inferior to DBD at high concentrations. However, DBD exceeded AR in vasodilating MCA at all concentrations. For U46619-constricted MCA, DBD, ASR, and AR had almost identical vasodilation. The dilation of DBD and AR on KCl-contracted MCA was independent of K channel blockers. However, ASR may inhibit the K channel opening partially through synergistic interactions with Gli and BaCl. DBD, ASR, and AR may be responsible for inhibiting [Ca], while ASR and AR can also inhibit [Ca].  DBD can relax MCA with no effects on resting vessels. The mechanism may be related to ASR's inhibition of K and K channels. Meanwhile, the inhibition of [Ca] by DBD, ASR, and AR as well as the inhibition of [Ca] by ASR and AR may contribute to dilate MCA.

Anticonstriction Effect of MCA in Rats by Danggui Buxue Decoction.

For thousands of years, the roots of  Pall (PLP) has been considered by traditional Chinese medicine as a drug that can improve mental or emotional disorders, including depression, anxiety and affective disorders. Unfortunately, the research on the mechanism of action and active ingredients of this beneficial drug is not comprehensive. This study focused on the activity of essential oil from PLP (EOP), systematically studied the antidepressant effect of EOP for the first time, and discussed the potential mechanism of its antidepressant effect. In this study, we used a mouse model of corticosterone (CORT)-induced depression, and found that EOP had a significant antidepressant effect on the symptoms of CORT-induced depression in mice, and significantly down-regulated the levels of CRH, ACTH and cortisol in the brain tissues of mice. In addition, we found that EOP treatment alleviated CORT-induced hippocampal neuron injury in mice  experiments. It was also found that EOP could inhibit CORT-induced apoptosis and improve the proliferation ability and cell viability of PC12 cells. Further, with the help of network analysis, it was revealed that PI3K-Akt might be one of the main signaling pathways of EOP against CORT-induced hippocampal neuron apoptosis. In this study, we also found that EOP up-regulated the phosphorylation of PI3K and Akt in CORT-induced mouse hippocampal neurons and PC12 cells, and promoted the nuclear transcription of Nrf2 in CORT-induced PC12 cells. In conclusion, with the integrated approach, we demonstrated that EOP exerted anti-apoptotic effects on hippocampal neurons through PI3K/Akt/Nrf2 signaling pathway.

Essential oil from the roots of  has protective effect against corticosterone-induced depression in mice via modulation of PI3K/Akt signaling pathway.

Danggui Buxue Decoction (DBD), a classic representative prescription of invigorating Qi and producing blood, is used to treat coronary heart disease angina pectoris and vascular injury diseases. Abnormal coronary artery is an important cause of cardiovascular disease. However, the mechanism of DBD dilates coronary arteries is still unclear.

ArticleTotal : 5	Year
Publication title Cited by 1	2022
Publication title Cited by 3	2023
Publication title	2024
Publication title	2024
Publication title	2025

Article	Year
Anticonstriction Effect of MCA in Rats by Danggui Buxue Decoction. Frontiers in pharmacology\| PubMed ID: 34867357	2021
Essential oil from the roots of has protective effect against corticosterone-induced depression in mice via modulation of PI3K/Akt signaling pathway. Frontiers in pharmacology\| PubMed ID: 36188568	2022
Danggui Buxue Decoction and its components dilate coronary artery through activating the inward rectification K channels pathway. Journal of ethnopharmacology\| PubMed ID: 39521101	2024

Pengmei Guo

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