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Minzu University of China

Key Laboratory of Ethnomedicine (Minzu University of China)

Renmin university of china

Catalpa sawdust, a promising biofuel production biomass, was pretreated by microwave-water, -NaOH, and -Ca(OH) to enhance enzymatic digestibility. After 48h enzymatic hydrolysis, microwave-Ca(OH) pretreated sample showed the highest reducing sugar yield. The content of hemicellulose and lignin in catalpa sawdust decreased after microwave-alkali pretreatment. SEM observation showed that the catalpa sawdust surface with microwave-Ca(OH) pretreatment suffered the most serious erosion. Crystallinity index of catalpa sawdust increased after all three kinds of pretreatment. The optimum conditions of microwave-Ca(OH) pretreatment were particle size of 40mesh, Ca(OH) dosage of 2.25% (w/v), microwave power of 400W, pretreatment time of 6min, enzyme loading of 175FPU/g, and hydrolysis time of 96h, and the reducing sugar yield of microwave-Ca(OH) pretreated catalpa sawdust reached 402.73mg/g, which increased by 682.15% compared with that of raw catalpa sawdust. The catalpa sawdust with microwave-Ca(OH) pretreatment is promising for biofuel production with great potential.

Microwave assisted alkaline pretreatment to enhance enzymatic saccharification of catalpa sawdust.

Rumen fluid, formed in rumen of ruminants, includes a complex microbial population of bacteria, protozoa, fungi and archaea, and has high ability to degrade lignocellulosic biomass. In this study, rumen fluid was used to ferment grass clipping for enhancing the hydrolysis and acidification of organic matters. Results showed that strict anaerobic condition, higher grass clipping content and smaller particle size of grass clipping were beneficial to the hydrolysis and acidification of organics. The increase of SCOD and total VFA concentration respectively reached 24.9 and 10.2 g/L with a suitable grass clipping content of 5%, a particle size <0.150 mm, and a fermentation time of 48 h. The VFA production was mainly attributed to the degradation of cellulose and hemicellulose with a total solid reduction of 55.7%. Firmicutes and Fibrobacteres were the major contributors to the degradation of cellulose and hemicellulose. The activity of carboxymethyl cellulose enzyme (CMCase), cellobiase and xylanase reached 0.027, 0.176 and 0.180 U/ml, respectively. The rumen fluid microorganisms successfully enhanced the hydrolysis and acidification of grass clipping.

Rumen fluid fermentation for enhancement of hydrolysis and acidification of grass clipping.

Ultrasound-calcium hydroxide (US-Ca(OH)) pretreatment effectively enhanced volatile fatty acid (VFA) production from lignocellulosic biomass. In this paper, solid and liquid fraction of pretreated grass clipping was for the first time separately fermented in order to improve organic recovery from liquid fraction and reduce inhibition due to alkaline pretreatment. The total VFA yield and VS removal reached 515 mg/g TS and 59.7% after solid-liquid separation, exhibiting an increase of 116.7% and 91.9% comparing to that of mixture sample. The dominate components of VFAs are acetic and propionate acid, accounting for 80-90% of total VFAs. Kinetic analysis showed that the highest maximum VFA production rate of 690 mg/L·d and the highest cumulative VFA production potential of 3299 mg/L were achieved in the fermentation of solid fraction. Microbial analysis showed that the dominate genera for VFA production were Halocella and Ruminiclostridium, both with a relative abundance of 20.1% in fermentation of solid fraction.

Benefit of solid-liquid separation on volatile fatty acid production from grass clipping with ultrasound-calcium hydroxide pretreatment.

High pressure homogenization (HPH) pretreatment can effectively enhance anaerobic sludge digestion. In order to understand the corresponding mechanisms, different homogenization pressures were applied on sewage sludge, and solid and liquid fractions were separately digested to clarify contribution of solid and liquid fractions to biogas production. Results showed that the methane was mainly produced from solid fraction, and methane yield was increased with the increase of pretreatment pressure. The biogas and methane production from sludge (digested without solid-liquid separation) was 17% and 45% higher than the sum of that from solid and liquid fractions (digested separately) under a pressure of 40 MPa, respectively. This indicated that the sludge liquid fraction synergistically improved the biodegradation of sludge solids. The improvement of anaerobic digestion was attributed to organic release by sludge disintegration, sludge disruption and further increase of particle surface area. The methane production was linear with effectiveness of HPH pretreatment.

Contribution of solid and liquid fractions of sewage sludge pretreated by high pressure homogenization to biogas production.

This work studied preparation of rice husk-based solid acid and its application for efficient hydrolysis and saccharification of corncob. Rice husk-based solid acid (RH-SOH) was prepared by one-step carbonization and sulfonation method. Analysis demonstrated that RH-SOH exhibited aromatic carbon sheets structure bearing -SOH, -COOH and -OH groups. The RH-SOH was then used to hydrolyse and saccharify corncob. Compared with solid acids made from activated carbon and microcrystalline cellulose, the RH-SOH showed the highest catalytic efficiency with the maximum reducing sugar yield of 486.53 mg/g and xylose of 253.03 mg/g, which was twice and five times higher than that of control, respectively. Its high efficiency was attributed to -OH and -COOH groups functioned synergistically with -SOH to hydrolyse lignocellulose by adsorbing β-1,4-glucan in corncob. This study provides a green and effective utilization technology of lignocellulosic biomass.

ArticleTotal : 3	Year
Publication title Cited by 5	2022
Publication title	2025
Publication title	2026

Article	Year
Microwave assisted alkaline pretreatment to enhance enzymatic saccharification of catalpa sawdust. Bioresource technology\| PubMed ID: 27631890	2016
Rumen fluid fermentation for enhancement of hydrolysis and acidification of grass clipping. Journal of environmental management\| PubMed ID: 29777997	2018
Benefit of solid-liquid separation on volatile fatty acid production from grass clipping with ultrasound-calcium hydroxide pretreatment. Bioresource technology\| PubMed ID: 30502607	2019
Contribution of solid and liquid fractions of sewage sludge pretreated by high pressure homogenization to biogas production. Bioresource technology\| PubMed ID: 31048265	2019
Rice husk-based solid acid for efficient hydrolysis and saccharification of corncob. Bioresource technology\| PubMed ID: 31386943	2019

Siqi Wang

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