production-modified-autologous-conditioned-serum-ex-vivo-assessment

Production of Modified Autologous Conditioned Serum and <em>Ex Vivo</em> Assessment of Its Healing Potential in Murine Corneal Epithelium

Human blood-derived topical therapies have been a boon to clinicians in recent decades. Autologous serum (AS) and platelet-rich plasma (PRP) are enriched ...

Chang Gung Memorial Hospital Linkou

Chang Gung University

National Yang Ming Chiao Tung University

Disease affecting the cornea is a common cause of blindness worldwide. To date, the amniotic membrane (AM) is the most widely used clinical method for cornea regeneration. However, donor-dependent differences in the AM may result in variable clinical outcomes. To overcome this issue, biomaterials are currently under investigation for corneal regeneration in vitro and in vivo. In this article, we highlight the recent advances in hydrogels, bioengineered prosthetic devices, contact lenses, and drug delivery systems for corneal regeneration. In clinical studies, the therapeutic effects of biomaterials, including fibrin and collagen-based hydrogels and silicone contact lenses, have been demonstrated in damaged cornea. The combination of cells and biomaterials may provide potential treatment in corneal wound healing in the future.

Applications of biomaterials in corneal wound healing.

To find clinical demographics of pterygium surgery and prevalence of conjunctival intraepithelial neoplasia (CIN) in pterygium specimen.

Clinical demographics of pterygium excision and prevalence of conjunctival intraepithelial neoplasia: a 15-year review.

To describe a case with a growing erythematous conjunctival mass in the left eye, diagnosed as conjunctival myxoma.

Clinical appearance and pathological findings of conjunctival myxoma: Case report and literature review.

To evaluate the role of Demodex infestation of the eyelids in patients with recurrent herpetic keratitis.

Potential Role and Significance of Ocular Demodicosis in Patients with Concomitant Refractory Herpetic Keratitis.

The purpose of this study is to present characteristics and topographic findings of patients with corneal ectasia and symptomatic ocular demodicosis.

Clinical characteristics and topographic findings of corneal ectasia in patients with symptomatic  blepharitis.

This study analyzed the clinical features and molecular characteristics of methicillin-susceptible  (MSSA) ocular infections in Taiwan and compared them between community-associated (CA) and health-care-associated (HA) infections. We collected  ocular isolates from patients at Chang Gung Memorial Hospital between 2010 and 2017. The infections were classified as CA or HA using epidemiological criteria, and the isolates were molecularly characterized using pulsed-field gel electrophoresis, multilocus sequence typing, and Panton-Valentine leukocidin (PVL) gene detection. Antibiotic susceptibility was evaluated using disk diffusion and an E test. A total of 104 MSSA ocular isolates were identified; 46 (44.2%) were CA-MSSA and 58 (55.8%) were HA-MSSA. Compared with HA-MSSA strains, CA-MSSA strains caused a significantly higher rate of keratitis, but a lower rate of conjunctivitis. We identified 14 pulsotypes. ST 7/pulsotype BA was frequently identified in both CA-MSSA (28.3%) and HA-MSSA (37.9%) cases. PVL genes were identified in seven isolates (6.7%). Both CA-MSSA and HA-MSSA isolates were highly susceptible to vancomycin, teicoplanin, tigecycline, sulfamethoxazole-trimethoprim, and fluoroquinolones. The most common ocular manifestations were keratitis and conjunctivitis for CA-MSSA and HA-MSSA, respectively. The MSSA ocular isolates had diverse molecular characteristics; no specific genotype differentiated CA-MSSA from HA-MSSA. Both strains exhibited similar antibiotic susceptibility.

Clinical Features and Molecular Characteristics of Methicillin-Susceptible  Ocular Infection in Taiwan.

The aim of this study was to evaluate the efficacy of a deep learning system in pterygium grading and recurrence prediction.

Application of a Deep Learning System in Pterygium Grading and Further Prediction of Recurrence with Slit Lamp Photographs.

We performed molecular identification and antifungal susceptibilities of pathogens and investigated clinical features of 43 culture-proven  keratitis cases from 2015-2020 in Taiwan. The pathogens were identified by sequencing of their internal transcribed spacer regions of ribosomal DNA and translation elongation factor 1α gene; their antifungal susceptibilities (to seven agents) were determined by broth microdilution method. We also collected clinical data to compare the drug susceptibilities and clinical features of  species complex (FSSC) isolates with those of other  species complexes (non-FSSC). The FSSC accounted for 76.7% pathogens, among which  (32.6%) and  (27.9%) were the most common species. Among clinically used antifungal agents, amphotericin B registered the lowest minimal inhibitory concentration (MIC), and the new azoles efinaconazole, lanoconazole and luliconazole, demonstrated even lower MICs against  species. The MICs of natamycin, voriconazole, chlorhexidine, lanoconazole, and luliconazole were higher for the FSSC than the non-FSSC, but no significant differences were noted in clinical outcomes, including corneal perforation and final visual acuity. In Taiwan, the FSSC was the most common complex in  keratitis; its MICs for five tested antifungal agents were higher than those of non-FSSC, but the clinical outcomes did not differ significantly.

 Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features.

 is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of  keratitis, and to explore the possible role of Panton-Valentine leucocidin (PVL), a major virulence factor of  We recruited 49 patients with culture-proven  keratitis between 2013 and 2017 at Chang Gung Memorial Hospital, Taiwan. PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCC), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion/E test. There were 49 patients with  keratitis; 17 (34.7%) were caused by methicillin-resistant  (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59/PFGE type D/SCC V/PVL (+). All methicillin-sensitive  (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA/MSSA or PVL(+)/PVL(-) groups. In Taiwan, approximately one third of  keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. Fluoroquinolones could be good empiric antibiotics for  keratitis.

 Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features.

To verify efficacy of automatic screening and classification of glaucoma with deep learning system.

Article	Year
Applications of biomaterials in corneal wound healing. Journal of the Chinese Medical Association : JCMA\| PubMed ID: 25455161	2015
Clinical demographics of pterygium excision and prevalence of conjunctival intraepithelial neoplasia: a 15-year review. International ophthalmology\| PubMed ID: 32198608	2020
Clinical appearance and pathological findings of conjunctival myxoma: Case report and literature review. American journal of ophthalmology case reports\| PubMed ID: 32803020	2020
Potential Role and Significance of Ocular Demodicosis in Patients with Concomitant Refractory Herpetic Keratitis. Clinical ophthalmology (Auckland, N.Z.)\| PubMed ID: 33380781	2020
Clinical characteristics and topographic findings of corneal ectasia in patients with symptomatic blepharitis. Taiwan journal of ophthalmology\| PubMed ID: 34295620	2021
Clinical Features and Molecular Characteristics of Methicillin-Susceptible Ocular Infection in Taiwan. Antibiotics (Basel, Switzerland)\| PubMed ID: 34943657	2021
Application of a Deep Learning System in Pterygium Grading and Further Prediction of Recurrence with Slit Lamp Photographs. Diagnostics (Basel, Switzerland)\| PubMed ID: 35453936	2022
Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features. Journal of fungi (Basel, Switzerland)\| PubMed ID: 35628732	2022
Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features. International journal of molecular sciences\| PubMed ID: 36233005	2022
Application of a deep learning system in glaucoma screening and further classification with colour fundus photographs: a case control study. BMC ophthalmology\| PubMed ID: 36510171	2022

Kuo-Hsuan Hung

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