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Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
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Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Article DOI: 10.3791/64911-v • 03:55 min • March 24th, 2023
March 24th, 2023 •
*1,2, *1,2, 3, 4,5, 2,6,7, 2,6
1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University
* These authors contributed equally

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Summary
Automatic Translation

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

Tags

Modified Autologous Conditioned Serum MACS Healing Potential Murine Corneal Epithelium Wound Healing Autologous Serum Standardized Preparation Process Cost Reduction Serum Eyedrops Patient Accessibility Affordable Treatment Option Wound Regeneration Tissue Regeneration Repair Anti-inflammation Ocular Surface Diseases
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