endothelialized-microfluidics-for-studying-microvascular-interactions

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Advances in  microfabrication techniques have enabled the production of inexpensive and  reproducible microfluidic systems for conducting biological and ...

Emory University School of Medicine

Georgia Institute of Technology and Emory University

Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta 

Winship Cancer Institute of Emory University

CYP1A1 y CYP1B1, los miembros de la familia de prote&#xED;nas del citocromo P450, est&#xE1;n regulados por esfuerzo de corte del fluido. Este estudio describe los efectos de la duraci&#xF3;n, magnitud y el patr&#xF3;n de esfuerzo cortante en la CYP1A1 y CYP1B1 expresiones en las c&#xE9;lulas endoteliales humanas, hacia la meta de la comprensi&#xF3;n del papel (s) de estos genes en las funciones celulares proaterog&#xE9;nicas o anti-aterog&#xE9;nicas endoteliales.

La expresi&#xF3;n de CYP1A1 y CYP1B1 en c&#xE9;lulas endoteliales humanas: el Reglamento por el esfuerzo cortante del fluido

CYP1A1&#x3068;CYP1B1&#x3001;&#x30B7;&#x30C8;&#x30AF;&#x30ED;&#x30E0;P450&#x30BF;&#x30F3;&#x30D1;&#x30AF;&#x8CEA;&#x30D5;&#x30A1;&#x30DF;&#x30EA;&#x30FC;&#x306E;&#x30E1;&#x30F3;&#x30D0;&#x30FC;&#x306F;&#x3001;&#x6D41;&#x4F53;&#x306E;&#x305B;&#x3093;&#x65AD;&#x5FDC;&#x529B;&#x306B;&#x3088;&#x3063;&#x3066;&#x898F;&#x5236;&#x3055;&#x308C;&#x3066;&#x3044;&#x308B;&#x3002;&#x672C;&#x7814;&#x7A76;&#x3067;&#x306F;&#x3001;&#x30D7;&#x30ED;&#x306E;&#x52D5;&#x8108;&#x786C;&#x5316;&#x3084;&#x6297;&#x52D5;&#x8108;&#x786C;&#x5316;&#x8840;&#x7BA1;&#x5185;&#x76AE;&#x7D30;&#x80DE;&#x306E;&#x6A5F;&#x80FD;&#x3067;&#x3053;&#x308C;&#x3089;&#x306E;&#x907A;&#x4F1D;&#x5B50;&#x306E;&#x5F79;&#x5272;&#xFF08;s&#xFF09;&#x3092;&#x7406;&#x89E3;&#x3059;&#x308B;&#x3053;&#x3068;&#x306E;&#x76EE;&#x6A19;&#x306B;&#x5411;&#x304B;&#x3063;&#x3066;&#x3001;&#x30D2;&#x30C8;&#x5185;&#x76AE;&#x7D30;&#x80DE;&#x306B;&#x304A;&#x3051;&#x308B;CYP1A1&#x3068;CYP1B1&#x5F0F;&#x306E;&#x305B;&#x3093;&#x65AD;&#x5FDC;&#x529B;&#x306E;&#x671F;&#x9593;&#x3001;&#x5927;&#x304D;&#x3055;&#x3068;&#x30D1;&#x30BF;&#x30FC;&#x30F3;&#x306E;&#x5F71;&#x97FF;&#x306B;&#x3064;&#x3044;&#x3066;&#x8AAC;&#x660E;&#x3057;&#x307E;&#x3059;&#x3002;

&#x30D2;&#x30C8;&#x5185;&#x76AE;&#x7D30;&#x80DE;&#x306B;&#x304A;&#x3051;&#x308B;CYP1A1&#x3068;CYP1B1&#x306E;&#x767A;&#x73FE;&#xFF1A;&#x6D41;&#x4F53;&#x305B;&#x3093;&#x65AD;&#x5FDC;&#x529B;&#x306B;&#x3088;&#x308B;&#x898F;&#x5236;

CYP1A1 &#x548C; CYP1B1&#xFF0C;&#x7EC6;&#x80DE;&#x8272;&#x7D20; p450 &#x6C27;&#x5316;&#x86CB;&#x767D;&#x5BB6;&#x65CF;&#xFF0C;&#x6210;&#x5458;&#x53D7;&#x6D41;&#x4F53;&#x7684;&#x526A;&#x5E94;&#x529B;&#x3002;&#x8FD9;&#x9879;&#x7814;&#x7A76;&#x5BF9; CYP1A1 &#x548C; CYP1B1 &#x8868;&#x8FBE;&#x5185;&#x76AE;&#x7EC6;&#x80DE;&#xFF0C;&#x5B9E;&#x73B0;&#x8FD9;&#x4E00;&#x76EE;&#x6807;&#x7684;&#x7406;&#x89E3;&#x8FD9;&#x4E9B;&#x57FA;&#x56E0;&#x5728; pro &#x7CA5;&#x6837;&#x6216; anti-atherogenic &#x7684;&#x5185;&#x76AE;&#x7EC6;&#x80DE;&#x529F;&#x80FD;&#x7684;&#x89D2;&#x8272;&#x63CF;&#x8FF0;&#x7684;&#x6301;&#x7EED;&#x65F6;&#x95F4;&#x3001; &#x4E25;&#x91CD;&#x7A0B;&#x5EA6;&#x548C;&#x526A;&#x5E94;&#x529B;&#x6A21;&#x5F0F;&#x7684;&#x5F71;&#x54CD;&#x3002;

CYP1A1 &#x548C; CYP1B1 &#x4EBA;&#x8840;&#x7BA1;&#x5185;&#x76AE;&#x7EC6;&#x80DE;&#x4E2D;&#x7684;&#x8868;&#x8FBE;&#xFF1A; &#x89C4;&#x7BA1;&#x6D41;&#x4F53;&#x526A;&#x5E94;&#x529B;&#x3002;

CYP1A1 and CYP1B1, members of the cytochrome P450 protein family, are regulated by fluid shear stress. This study describes the effects of duration, magnitude and pattern of shear stress on CYP1A1 and CYP1B1 expressions in human endothelial cells, towards the goal of understanding the role(s) of these genes in pro-atherogenic or anti-atherogenic endothelial cell functions.

Expression of CYP1A1 and CYP1B1 in human endothelial cells: regulation by fluid shear stress.

CYP1A1 et CYP1B1, les membres de la famille des prot&#xE9;ines du cytochrome P450, sont r&#xE9;glement&#xE9;s par la contrainte de cisaillement du fluide. Cette &#xE9;tude d&#xE9;crit les effets de la dur&#xE9;e, l&#x26;#39;ampleur et le type de contrainte de cisaillement sur les CYP1A1 et CYP1B1 expressions dans les cellules endoth&#xE9;liales humaines, vers le but de comprendre le r&#xF4;le (s) de ces g&#xE8;nes dans pro-ath&#xE9;rog&#xE8;nes et anti-ath&#xE9;rog&#xE8;ne fonctions des cellules endoth&#xE9;liales.

L&#x26;#39;expression de CYP1A1 et le CYP1B1 cellules endoth&#xE9;liales humaines: le r&#xE8;glement par le stress de cisaillement du fluide

CYP1A1 und CYP1B1, Mitglieder des Cytochrom P450-Proteinfamilie, fl&uuml;ssig Schubspannung geregelt. Diese Studie beschreibt die Auswirkungen der Dauer, Ausma&szlig; und Muster der Scherspannung CYP1A1 und CYP1B1-Ausdr&uuml;cke in menschlichen Endothelzellen, in Richtung des Ziels die Rolle(n) dieser Gene in Pro-atherogenic oder anti-atherogenic Endothelzellen Funktionen zu verstehen.

Ausdruck von CYP1A1 und CYP1B1 in menschlichen Endothelzellen: Verordnung von Fl&uuml;ssigkeit Schubspannung.

CYP1A1 e CYP1B1, membri del citocromo P450 famiglia di proteine, sono regolati dal fluido shear stress. Questo studio descrive gli effetti di durata, grandezza e modello di shear stress sulle espressioni CYP1A1 e CYP1B1 in cellule endoteliali umane, verso l&#39;obiettivo di comprendere i ruoli di questi geni in funzioni delle cellule endoteliali pro-aterogeno o anti-atherogenic.

Espressione di CYP1A1 e CYP1B1 in cellule endoteliali umane: regolamento di fluido shear stress.

CYP1A1 &#xBC0F; CYP1B1, &#xC2DC; &#xD1A0; &#xD06C;&#xB86C; P450 &#xB2E8;&#xBC31;&#xC9C8; &#xAC00;&#xC871;&#xC758; &#xAD6C;&#xC131;&#xC6D0;&#xC740; &#xC720;&#xCCB4; &#xC804;&#xB2E8; &#xC751;&#xB825;&#xC5D0; &#xC758;&#xD574; &#xADDC;&#xC81C; &#xB429;&#xB2C8;&#xB2E4;. &#xC774; &#xC5F0;&#xAD6C;&#xB294; &#xD504;&#xB85C; atherogenic &#xB610;&#xB294; anti-atherogenic &#xB0B4; &#xD53C; &#xC138;&#xD3EC; &#xAE30;&#xB2A5;&#xC5D0;&#xC11C;&#xC774; &#xC720;&#xC804;&#xC790;&#xC758; &#xC5ED;&#xD560;&#xC744; &#xC774;&#xD574; &#xD558;&#xB294; &#xBAA9;&#xD45C;&#xB97C; &#xD5A5;&#xD574; &#xC778;&#xAC04; &#xB0B4; &#xD53C; &#xC138;&#xD3EC;&#xC5D0;&#xC11C; CYP1A1 &#xBC0F; CYP1B1 &#xC2DD;&#xC5D0; &#xAE30;&#xAC04;, &#xADDC;&#xBAA8;&#xC640; &#xC804;&#xB2E8; &#xC751;&#xB825;&#xC758; &#xD328;&#xD134;&#xC758; &#xC601;&#xD5A5;&#xC744; &#xC124;&#xBA85; &#xD569;&#xB2C8;&#xB2E4;.

CYP1A1 &#xBC0F; &#xC778;&#xAC04;&#xC758; &#xB0B4; &#xD53C; &#xC138;&#xD3EC;&#xC5D0;&#xC11C; cyp1b1&#xC758; &#xD45C;&#xD604;: &#xC720;&#xCCB4; &#xC804;&#xB2E8; &#xC751;&#xB825;&#xC5D0;&#xC758; &#xD55C; &#xADDC;&#xC81C;.

To activate clot formation and maintain hemostasis, platelets adhere and spread onto sites of vascular injury. Although this process is well-characterized biochemically, how the physical and spatial cues in the microenvironment affect platelet adhesion and spreading remain unclear. In this study, we applied deep UV photolithography and protein micro/nanostamping to quantitatively investigate and characterize the spatial guidance of platelet spreading at the single cell level and with nanoscale resolution. Platelets adhered to and spread only onto micropatterned collagen or fibrinogen surfaces and followed the microenvironmental geometry with high fidelity and with single micron precision. Using micropatterned lines of different widths, we determined that platelets are able to conform to micropatterned stripes as thin as 0.6 µm and adopt a maximum aspect ratio of 19 on those protein patterns. Interestingly, platelets were also able to span and spread over non-patterned regions of up to 5 µm, a length consistent with that of maximally extended filopodia. This process appears to be mediated by platelet filopodia that are sensitive to spatial cues. Finally, we observed that microenvironmental geometry directly affects platelet biology, such as the spatial organization and distribution of the platelet actin cytoskeleton. Our data demonstrate that platelet spreading is a finely-tuned and spatially-guided process in which spatial cues directly influence the biological aspects of how clot formation is regulated.

Article	Year
Analytical biochemistry\| PubMed ID: 12672423	2003
Veterinary immunology and immunopathology\| PubMed ID: 15010227	2004
The American journal of pathology\| PubMed ID: 15111303	2004
Annals of biomedical engineering\| PubMed ID: 16341922	2005
Annals of biomedical engineering\| PubMed ID: 16565786	2006
Endothelium : journal of endothelial cell research\| PubMed ID: 18568947	2008
Expression of CYP1A1 and CYP1B1 in human endothelial cells: regulation by fluid shear stress. Cardiovascular research\| PubMed ID: 19126602	2009
Journal of leukocyte biology\| PubMed ID: 19706840	2009
Journal of vascular research\| PubMed ID: 19729955	2010
Immunology\| PubMed ID: 19950419	2009
Microenvironmental geometry guides platelet adhesion and spreading: a quantitative analysis at the single cell level. PloS one\| PubMed ID: 22028878	2011

Yumiko Sakurai

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