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Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
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11:08 min
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June 22, 2012
DOI:
1Department of Pediatrics,Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children’s Healthcare of Atlanta , 4Winship Cancer Institute of Emory University
Summary
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A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.
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MicrofluidicsEndothelialized MicrofluidicsMicrovascular InteractionsHematologic DiseasesMicrofabrication TechniquesBiological ExperimentsBiochemical ExperimentsMicro- And NanoscalesHematologic ProcessesBlood Cell DeformabilityBlood Cell AggregationMicrovascular Blood FlowBlood Cell-endothelial Cell InteractionsCultured Endothelial CellsMicrocirculationBiophysical Pathophysiology
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Cite this Article
Myers, D. R., Sakurai, Y., Tran, R., Ahn, B., Hardy, E. T., Mannino, R., Kita, A., Tsai, M., Lam, W. A. Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases. J. Vis. Exp. (64), e3958, doi:10.3791/3958 (2012).
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