Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

David R. Myers; Yumiko Sakurai; Reginald Tran; Byungwook Ahn; Elaissa Trybus Hardy; Robert Mannino; Ashley Kita; Michelle Tsai; Wilbur A. Lam

doi:10.3791/3958

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

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11:08 min

June 22, 2012

DOI: 10.3791/3958-v

David R. Myers*^1,2,3,4, Yumiko Sakurai*^1,2,3,4, Reginald Tran^1,2,3,4, Byungwook Ahn^1,2,3,4, Elaissa Trybus Hardy^1,2,3,4, Robert Mannino^1,2,3,4, Ashley Kita^1,2,3,4, Michelle Tsai^1,2,3,4, Wilbur A. Lam^1,2,3,4

¹Department of Pediatrics,Emory University School of Medicine, ²Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, ³Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta , ⁴Winship Cancer Institute of Emory University

A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.

The overall goal of this procedure is to create a microfluidic device coated with endothelial cells. This is accomplished by first fabricating the microfluidic mold. The second step is to create the PDMS channel.

Next, the endothelial cells are seeded into the device. The final step is to culture the cells within the device. Ultimately, endothelial cell coated microfluidic devices are used to show cell to cell interactions in vitro.

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