isolation-and-culture-of-rat-embryonic-neural-cells-a-quick-protocol

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

We are describing a quick method to dissociate and culture hippocampal or cortical neurons from E15-17 rat embryos. The procedure can be applied ...

cerebrospinal-fluid-microrna-profiling-using-quantitative-real-time

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

MicroRNAs (miRNAs) constitute a potent layer of gene regulation by guiding RISC to target sites located on mRNAs and, consequently, by modulating their ...

LSU Health Sciences Center - New Orleans

Medical School and Stanley S. Scott Cancer Center

LSU Health Sciences Center

MicroRNAs are short non-coding RNAs that modulate gene expression by translational repression. Because of their high stability in intracellular as well as extracellular environments, miRNAs have recently emerged as important biomarkers in several human diseases. However, they have not been tested in the cerebrospinal fluid (CSF) of HIV-1 positive individuals. Here, we present results of a study aimed at determining the feasibility of detecting miRNAs in the CSF of HIV-infected individuals with and without encephalitis (HIVE). We also evaluated similarities and differences between CSF and brain tissue miRNAs in the same clinical setting. We utilized a high throughput approach of miRNA detection arrays and identified differentially expressed miRNAs in the frontal cortex of three cases each of HIV+, HIVE, and HIV- controls, and CSF of 10 HIV-positive and 10 HIV-negative individuals. For the CSF samples, the group of HIV+ individuals contained nine cases of HIV-associated neurological disorders (HAND) and, among those, four had HIVE. All the HIV-negative samples had non-viral acute disseminate encephalomyelitis. A total of 66 miRNAs were found differentially regulated in HIV+ compared to HIV- groups. The greatest difference in miRNA expression was observed when four cases of HIVE were compared to five non-HIVE cases, previously normalized with the HIV-negative group. After statistical analyses, 11 miRNAs were fund significantly up-regulated in HIVE. Although more clinical samples should be examined, this work represents the first report of CSF miRNAs in HIV-infection and offers the basis for future investigation.

ArticleTotal : 2	Year
Publication title Cited by 79	2012
Publication title Cited by 10	2014

Article	Year
FASEB journal : official publication of the Federation of American Societies for Experimental Biology\| PubMed ID: 20181935	2010
Journal of cellular physiology\| PubMed ID: 21506108	2011
Biochimica et biophysica acta\| PubMed ID: 21745501	2011
Cerebrospinal fluid miRNA profile in HIV-encephalitis. Journal of cellular physiology\| PubMed ID: 23042033	2013

Marco Pacifici

Biography

JoVE Journal Publications

Other Publications