exploring-pharmacological-action-molecular-mechanism-salidroside

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Salidroside (Sal) contains anti-carcinogenic, anti-hypoxic, and anti-inflammatory pharmacological activities. However, its underlying anti-breast cancer ...

dynamic-changes-lung-function-blood-flow-during-lung-adenocarcinoma

Dynamic Changes in Lung Function and Blood Flow During Lung Adenocarcinoma Progression in Mice by Whole-body Plethysmography and Doppler Ultrasound

Lung adenocarcinoma (LUAD) is a common malignant pulmonary tumor, and its progression leads to alterations in ventilation and blood flow, highlighting the ...

Suining Central Hospital

China Civil Affairs University

Adenomatoid tumor of the fallopian tube.

With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels of at least 0.50 mg/dl or an increase >25% of serum creatinine from baseline after exposure to contrast. In this study, the protective effects of salvianolic acid B (Sal B) were detected in human renal tubular epithelial cells (HK-2) exposed to iopromide. The results showed that different concentrations of Sal B counteract the loss of cell viability induced by iopromide, and reduce cell apoptosis, the reactive oxygen species (ROS) levels, and the levels of endoplasmic reticulum stress (ERS)-related and apoptosis-related proteins such as p-IRE-1α, p-eIF-2α/eIF-2α, p-JNK, CHOP, Bax/Bcl-2, and cleaved caspase-3. In addition, Sal B at a concentration of 100 μmol/L inhibited ERS and reduced cell damage to a similar extent as the ERS inhibitor 4-PBA. Importantly, treatment with Sal B could abolish the injury induced by ERS agonist tunicamycin, increasing cell viability and the mitochondrial membrane potential, as well as significantly reducing ROS levels and the expression of Bax/Bcl-2, cleaved-caspase-3, GRP78, p-eIF2α, p-JNK, and CHOP. These results suggested that the protective effect of Sal B against HK-2 cell injury induced by iopromide may be related to the inhibition of ERS.

Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide.

Encapsulated papillary carcinoma with infiltrating ductal carcinoma in a male breast.

Postcontrast acute kidney injury (PC-AKI) is directly caused by the use of contrast, indicating a clear causal relationship between the contrast and the injury. Salvianolic acid B (Sal B), a water-soluble compound of , has a potent anti-inflammatory effect. We conducted a study to explore whether the protective effect of Sal B on iopromide-induced injury in human proximal tubular epithelial cells (HK-2 cells) is related to inhibition of the TLR4/NF-B/NLRP3 signal pathway. The results showed that 100 mol/L Sal B counteracted the decrease in cell viability, the increase of ROS and the number of apoptotic cells, and the decrease of mitochondrial membrane potential (ΔΨm) induced by iopromide. Molecular docking analysis showed that Sal B binds TLR4 and NLRP3 proteins. Moreover, 100 mol/L Sal B also decreased the expression of TLR4, NLRP3, ASC, Caspase-1, IL-18, IL-1, TNF-, p-NF-B, cleaved caspase-3, and the ratio of Bax/Bcl-2 induced by iopromide. TAK-242, a TLR4 antagonist, was added to further explore the mechanism of Sal B. However, the cotreatment group with TAK-242 and Sal B had no significant difference in cell viability and apoptosis rate compared to the treatment group with TAK-242 or Sal B alone. These results indicated that Sal B can inhibit the TLR4/NF-B/NLRP3 signal pathway, resulting in the alleviation of iopromide-induced HK-2 cell injury.

ArticleTotal : 2	Year
Publication title Cited by 4	2023
Publication title	2025

Article	Year
Adenomatoid tumor of the fallopian tube. Asian journal of surgery\| PubMed ID: 34955345	2022
Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide. Frontiers in pharmacology\| PubMed ID: 35035354	2021
Encapsulated papillary carcinoma with infiltrating ductal carcinoma in a male breast. Asian journal of surgery\| PubMed ID: 35304053	2022
Salvianolic Acid B Attenuates Iopromide-Induced Renal Tubular Epithelial Cell Injury by Inhibiting the TLR4/NF-B/NLRP3 Signaling Pathway. Evidence-based complementary and alternative medicine : eCAM\| PubMed ID: 35795279	2022
Intrathyroid branchial-cleft cyst. Asian journal of surgery\| PubMed ID: 36117065	2023
Lymphangioma of the esophagus. Asian journal of surgery\| PubMed ID: 36184288	2023
Myxoid adrenocortical adenoma. Asian journal of surgery\| PubMed ID: 36891757	2023
Cervical mixed adenocarcinoma-neuroendocrine carcinoma: A case report with liquid-based cytology. Asian journal of surgery\| PubMed ID: 37002042	2023

Ting Luo

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